New pre-attachment Striga resistant sorghum adapted to African agro-ecologies.
Striga hermonthica
lgs1
low germination stimulant 1
parasitic plants
pre-attachment resistance and radicle length
sorghum
Journal
Pest management science
ISSN: 1526-4998
Titre abrégé: Pest Manag Sci
Pays: England
ID NLM: 100898744
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
revised:
23
01
2021
received:
02
09
2020
accepted:
12
02
2021
pubmed:
13
2
2021
medline:
18
5
2021
entrez:
12
2
2021
Statut:
ppublish
Résumé
Pre-attachment resistance to the parasitic plants Striga hermonthica and S. asiatica occurs in sorghum mutants designated low germination stimulant 1 (lgs1). However, only a few of these mutants have been identified and their resistance validated. Additionally, pre-attachment resistance in sorghum beyond lgs1 mutants has not been explored. We used lgs1-specific markers to identify new lgs1-like mutants in a diverse global sorghum collection. The sorghum collection was also evaluated for pre-attachment resistance against Striga using an in vitro assay that measured Striga germination activity and radicle growth. From a total of 177 sorghum accessions, 60 recorded mean germination levels of below 42%, which is comparable with the previously identified lgs1-like sorghum (SRN39 and IS9830) used as controls in this study. Furthermore, 32 of these accessions recorded Striga radicle lengths comparable or lower than the controls (0.42 mm). Thirty-eight accessions contained the lgs1 mutation and although overall, lgs1 mutants had considerably reduced Striga germination, some low inducers of Striga germination were wild-type for lgs1. Germination was positively but weakly correlated with radicle length pointing to additional radicle growth inhibitory activity. lgs1 mutations, alongside other mechanisms for low Striga germination stimulation, are prevalent in sorghum, and poor Striga radicle growth is suggestive of host-derived inhibition. As an outcome, our study makes available multiple Striga-resistant sorghum with adaptability to diverse agro-ecological regions in sub-Saharan Africa making immediate deployment possible. © 2021 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Pre-attachment resistance to the parasitic plants Striga hermonthica and S. asiatica occurs in sorghum mutants designated low germination stimulant 1 (lgs1). However, only a few of these mutants have been identified and their resistance validated. Additionally, pre-attachment resistance in sorghum beyond lgs1 mutants has not been explored. We used lgs1-specific markers to identify new lgs1-like mutants in a diverse global sorghum collection. The sorghum collection was also evaluated for pre-attachment resistance against Striga using an in vitro assay that measured Striga germination activity and radicle growth.
RESULTS
RESULTS
From a total of 177 sorghum accessions, 60 recorded mean germination levels of below 42%, which is comparable with the previously identified lgs1-like sorghum (SRN39 and IS9830) used as controls in this study. Furthermore, 32 of these accessions recorded Striga radicle lengths comparable or lower than the controls (0.42 mm). Thirty-eight accessions contained the lgs1 mutation and although overall, lgs1 mutants had considerably reduced Striga germination, some low inducers of Striga germination were wild-type for lgs1. Germination was positively but weakly correlated with radicle length pointing to additional radicle growth inhibitory activity.
CONCLUSIONS
CONCLUSIONS
lgs1 mutations, alongside other mechanisms for low Striga germination stimulation, are prevalent in sorghum, and poor Striga radicle growth is suggestive of host-derived inhibition. As an outcome, our study makes available multiple Striga-resistant sorghum with adaptability to diverse agro-ecological regions in sub-Saharan Africa making immediate deployment possible. © 2021 Society of Chemical Industry.
Substances chimiques
Lactones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2894-2902Subventions
Organisme : International Centre for Genetic Engineering and Biotechnology
ID : CRP/18/001
Organisme : United States Agency for International Development
ID : PGA- 2000008288
Informations de copyright
© 2021 Society of Chemical Industry.
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