Transient systemic inflammation in adult male mice results in underweight progeny.
Animals
Cytokines
/ metabolism
Histone Methyltransferases
/ genetics
Immune Privilege
Infertility, Male
/ immunology
Inflammation
/ immunology
Inflammation Mediators
/ metabolism
Lipopolysaccharides
/ immunology
Male
Mice
Mice, Inbred C57BL
Myeloid-Lymphoid Leukemia Protein
/ genetics
Orchitis
/ immunology
Peptidoglycan
/ immunology
Poly I-C
/ immunology
Testis
/ metabolism
Thinness
/ immunology
epigenetics
inflammation
male fertility
placenta
progeny
sperm
Journal
American journal of reproductive immunology (New York, N.Y. : 1989)
ISSN: 1600-0897
Titre abrégé: Am J Reprod Immunol
Pays: Denmark
ID NLM: 8912860
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
received:
22
12
2020
accepted:
09
02
2021
pubmed:
13
2
2021
medline:
27
1
2022
entrez:
12
2
2021
Statut:
ppublish
Résumé
While the testes represent an immune-privileged organ, there is evidence that systemic inflammation is accompanied by local inflammatory responses. We therefore examined whether transient systemic inflammation caused any inflammatory and functional consequences in murine testes. Using a single systemic administration of Toll-like receptor (TLR) agonists [lipopolysaccharide (LPS) or peptidoglycan (PG) or polyinosinic-polycytidylic acid (polyIC)] in young adult male mice, we assessed testicular immune-inflammatory landscape and reproductive functionality. Our findings demonstrated a significant induction of testicular TNF-α, IL-1β and IL-6 transcripts within 24 h of TLR agonist injection. By day 6, these cytokine levels returned to baseline. While there was no change in caudal sperm counts at early time points, eight weeks later, twofold decrease in sperm count and reduced testicular testosterone levels were evident. When these mice were subjected to mating studies, no differences in mating efficiencies or litter sizes were observed compared with controls. Nonetheless, the neonatal weights of progeny from LPS/PG/polyIC-treated sires were significantly lower than controls. Postnatal weight gain up to three weeks was also slower in the progeny of LPS/polyIC-treated sires. Placental weights at 17.5 days post-coitum were significantly lower in females mated to LPS- and polyIC-treated males. Given this likelihood of an epigenetic effect, we found lower testicular levels of histone methyltransferase enzyme, mixed-lineage leukaemia-1, in mice given LPS/PG/polyIC 8 weeks earlier. Exposure to transient systemic inflammation leads to transient local inflammation in the testes, with persistent sperm-mediated consequences for foetal development.
Substances chimiques
Cytokines
0
Inflammation Mediators
0
Lipopolysaccharides
0
Peptidoglycan
0
Myeloid-Lymphoid Leukemia Protein
149025-06-9
Histone Methyltransferases
EC 2.1.1.-
Poly I-C
O84C90HH2L
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13401Informations de copyright
© 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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