Bioinformatic Tools and Guidelines for the Design of Fluorescence In Situ Hybridization Probes.
2’OMe
Bioinformatic tools
DNA
Fluorescence in situ hybridization
LNA
Nucleic Acid Mimics
PNA
Probe design
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
12
2
2021
pubmed:
13
2
2021
medline:
31
3
2021
Statut:
ppublish
Résumé
Fluorescence in situ hybridization (FISH) is a well-established technique that allows the detection of microorganisms in diverse types of samples (e.g., clinical, food, environmental samples, and biofilm communities). The FISH probe design is an essential step in this technique. For this, two strategies can be used, the manual form based on multiple sequence alignment to identify conserved regions and programs/software specifically developed for the selection of the sequence of the probe. Additionally, databases/software for the theoretical evaluation of the probes in terms of specificity, sensitivity, and thermodynamic parameters (melting temperature and Gibbs free energy change) are used. The purpose of this chapter is to describe the essential steps and guidelines for the design of FISH probes (e.g., DNA and Nucleic Acid Mimic (NAM) probes), and its theoretical evaluation through the application of diverse bioinformatic tools.
Identifiants
pubmed: 33576981
doi: 10.1007/978-1-0716-1115-9_3
doi:
Substances chimiques
Nucleic Acids
0
Oligonucleotide Probes
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
35-50Références
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