Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization.
2’OMe
FISH
LNA
Microorganisms
Nucleic acid mimics
PNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
12
2
2021
pubmed:
13
2
2021
medline:
31
3
2021
Statut:
ppublish
Résumé
Traditionally, RNA and DNA probes are used in fluorescence in situ hybridization (FISH) methods for microbial detection and characterization of communities' structure and diversity. However, the recent introduction of nucleic acid mimics (NAMs) has improved the robustness of the FISH methods in terms of sensitivity and specificity. Several NAMs have been used, of which the most relevant are peptide nucleic acid (PNA), locked nucleic acids (LNA), 2'-O-methyl RNA (2'OMe), and phosphorothioates (PS). In this chapter, we describe a protocol using PNA and LNA/2'OMe probes for microbial detection by FISH, pointing out the differences between them. These protocols are easily adapted to different microorganisms and different probe sequences.
Identifiants
pubmed: 33576983
doi: 10.1007/978-1-0716-1115-9_5
doi:
Substances chimiques
Nucleic Acid Probes
0
Nucleic Acids
0
Oligonucleotides
0
Peptide Nucleic Acids
0
locked nucleic acid
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-86Références
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