Dietary tryptophan depletion alters the faecal bacterial community structure of compulsive drinker rats in schedule-induced polydipsia.

Animals Diet Polydipsia RNA, Ribosomal, 16S / genetics Rats Rats, Wistar Tryptophan
Brain-derived neurotrophic factor Chronic tryptophan depletion Compulsivity Gut microbiota PCR-DGGE Schedule-induced polydipsia

Journal

Physiology & behavior
ISSN: 1873-507X
Titre abrégé: Physiol Behav
Pays: United States
ID NLM: 0151504

Informations de publication

Date de publication:
01 05 2021
Historique:
received: 25 10 2020
revised: 29 01 2021
accepted: 09 02 2021
pubmed: 13 2 2021
medline: 29 6 2021
entrez: 12 2 2021
Statut: ppublish

Résumé

Compulsive behaviour, present in different psychiatric disorders such as obsessive-compulsive disorder, schizophrenia and drug abuse, is associated with altered levels of serotonin (5-hydroxytryptamine, 5-HT). The gut microbiota regulates tryptophan (TRP) metabolism and may affect global 5-H synthesis in the enteric and central nervous systems, suggesting a possible involvement of gut microbiota in compulsive spectrum disorders. The present study investigated whether chronic TRP depletion by diet alters the faecal bacterial community profiles of compulsive versus non-compulsive rats in schedule-induced polydipsia (SIP). Peripheral plasma 5-HT and brain-derived neurotrophic factor (BDNF) levels were evaluated. Wistar rats were selected as High Drinkers (HD) or Low Drinkers (LD) according to their SIP behaviour and were fed for 14 days with either a TRP-free diet (T-) or a TRP-supplemented diet (T+). The faecal bacterial community structure was investigated with 16S rRNA gene-targeted denaturing gradient gel electrophoresis (DGGE) fingerprinting analysis. Compulsive HD rats showed a lower bacterial diversity than LD rats, irrespectively of the diet. The TRP-depleted HD rats, the only group increasing compulsive licking in SIP, showed a reduction of bacterial evenness and a highly functionally organized community compared with the other groups, indicating that this bacterial community is more fragile to external changes due to the dominance of a low number of species. The chronic TRP depletion by diet effectively reduced peripheral plasma 5-HT levels in both HD and LD rats, while plasma BDNF levels were not altered. These results highlight the possible implication of reduced microbial diversity in compulsive behaviour and the involvement of the serotonergic system in modulating the gut brain-axis in compulsive spectrum disorders.

Identifiants

DOI: 10.1016/j.physbeh.2021.113356 PMID: 33577871
pubmed: 33577871
pii: S0031-9384(21)00048-2
doi: 10.1016/j.physbeh.2021.113356
pii:
doi:

Substances chimiques

RNA, Ribosomal, 16S 0
Tryptophan 8DUH1N11BX

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113356

Informations de copyright

Copyright © 2021. Published by Elsevier Inc.

Auteurs

A Merchán (A)

Department of Psychology and Health Research Center (CEINSA), University of Almería, Ctra. Sacramento s/n, 04120 Almería, Spain.

C Pérez-Fernández (C)

Department of Psychology and Health Research Center (CEINSA), University of Almería, Ctra. Sacramento s/n, 04120 Almería, Spain.

M J López (MJ)

Department of Biology and Geology and CIAMBITAL, University of Almería & CeiA3, Ctra. Sacramento s/n, 04120, Almería, Spain.

J Moreno (J)

Department of Biology and Geology and CIAMBITAL, University of Almería & CeiA3, Ctra. Sacramento s/n, 04120, Almería, Spain.

M Moreno (M)

Department of Psychology and Health Research Center (CEINSA), University of Almería, Ctra. Sacramento s/n, 04120 Almería, Spain.

F Sánchez-Santed (F)

Department of Psychology and Health Research Center (CEINSA), University of Almería, Ctra. Sacramento s/n, 04120 Almería, Spain.

P Flores (P)

Department of Psychology and Health Research Center (CEINSA), University of Almería, Ctra. Sacramento s/n, 04120 Almería, Spain. Electronic address: pflores@ual.es.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Dietary tryptophan depletion alters the faecal...
questionsmedicales.fr › Phénomènes physiologiques › Alimentation et nutrition › Phénomènes physiologiques nutritionnels › Régime alimentaire › Dietary tryptophan depletion alters the faecal...
questionsmedicales.fr › États, signes et symptômes pathologiques › Signes et symptômes › Polydipsie › Dietary tryptophan depletion alters the faecal...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Dietary tryptophan depletion alters the faecal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Dietary tryptophan depletion alters the faecal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Rat Wistar › Dietary tryptophan depletion alters the faecal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés essentiels › Tryptophane › Dietary tryptophan depletion alters the faecal...