Interaction of implant infection-related commensal bacteria with mesenchymal stem cells: a comparison between Cutibacterium acnes and Staphylococcus aureus.


Journal

FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721

Informations de publication

Date de publication:
19 03 2021
Historique:
received: 11 11 2020
accepted: 11 02 2021
pubmed: 14 2 2021
medline: 25 2 2023
entrez: 13 2 2021
Statut: ppublish

Résumé

Staphylococcus aureus and Cutibacterium acnes are involved in several tissue infections and can encounter mesenchymal stem cells (MSCs) during their role in tissue regenerative process. C. acnes and S. aureus internalization by three types of MSCs derived from bone marrow, dental pulp and Wharton's jelly; and bacterial biofilm production were compared. Internalization rates ranged between 1.7-6.3% and 0.8-2.7% for C. acnes and S. aureus, respectively. While C. acnes strains exhibited limited cytotoxic effect on MSCs, S. aureus were more virulent with marked effect starting after only 3 h of interaction. Both bacteria were able to produce biofilms with respectively aggregated and monolayered structures for C. acnes and S. aureus. The increase in C. acnes capacity to develop biofilm following MSCs' internalization was not linked to the significant increase in number of live bacteria, except for bone marrow-MSCs/C. acnes CIP 53.117 with 79% live bacteria compared to the 36% before internalization. On the other hand, internalization of S. aureus had no impact on its ability to form biofilms composed mainly of living bacteria. The present study underlined the complexity of MSCs-bacteria cross-interaction and brought insights into understanding the MSCs behavior in response to bacterial infection in tissue regeneration context.

Identifiants

pubmed: 33580963
pii: 6134754
doi: 10.1093/femsle/fnab014
pii:
doi:

Types de publication

Comparative Study Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS.

Auteurs

Taghrid S El-Mahdy (TS)

Department of Microbiology and Immunology, Faculty of Pharmacy, Helwan University, Cairo, Egypt.
Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Cairo, Egypt.

Céline Mongaret (C)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Université de Reims Champagne-Ardenne, UFR de Pharmacie, 51100 Reims, France.
CHU Reims, Service Pharmacie, France.

Jennifer Varin-Simon (J)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.

Fabien Lamret (F)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.

Véronique Vernet-Garnier (V)

CHU Reims, Laboratoire de Bactériologie-Hygiène, France.

Hassan Rammal (H)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Université de Reims Champagne-Ardenne, UFR d'Odontologie, 51100 Reims, France.

Cedric Mauprivez (C)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Université de Reims Champagne-Ardenne, UFR d'Odontologie, 51100 Reims, France.

Halima Kerdjoudj (H)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Université de Reims Champagne-Ardenne, UFR d'Odontologie, 51100 Reims, France.

Sophie C Gangloff (SC)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Université de Reims Champagne-Ardenne, UFR de Pharmacie, 51100 Reims, France.

Fany Reffuveille (F)

Université de Reims Champagne-Ardenne, EA 4691, Biomatériaux et inflammation en site osseux (BIOS), Reims, France.
Université de Reims Champagne-Ardenne, UFR de Pharmacie, 51100 Reims, France.

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Classifications MeSH