Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-Tag Labeling Approach.
ABC transporter
His-tag labeling
Membrane proteins
MicroScale thermophoresis58
RED-tris-NTA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
14
2
2021
pubmed:
15
2
2021
medline:
1
4
2021
Statut:
ppublish
Résumé
The combination of MicroScale Thermophoresis (MST) and near-native site-specific His-tag labeling enables simple, robust, and reliable determination of the binding affinity between proteins and ligands. To demonstrate its applicability for periplasmic proteins, we provide a detailed protocol for determination of the binding affinity of phosphite to three ABC transporter periplasmic-binding proteins from environmental microorganisms. ABC transporters are central to many important biomedical phenomena, including resistance of cancers and pathogenic microbes to drugs. The protocol described here can be used to quantify protein-ligand and protein-protein interactions for other soluble, membrane-associated and integral membrane proteins.
Identifiants
pubmed: 33582986
doi: 10.1007/978-1-0716-0724-4_2
doi:
Substances chimiques
ATP-Binding Cassette Transporters
0
Ligands
0
Periplasmic Binding Proteins
0
Phosphites
0
Histidine
4QD397987E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
51-62Références
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