Pold4, the fourth subunit of replicative polymerase δ, suppresses gene conversion in the immunoglobulin-variable gene in avian DT40 cells.
DNA damage
Gene conversion
Homologous recombination
Replication
Translesion DNA synthesis
Journal
DNA repair
ISSN: 1568-7856
Titre abrégé: DNA Repair (Amst)
Pays: Netherlands
ID NLM: 101139138
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
22
12
2020
revised:
26
01
2021
accepted:
28
01
2021
pubmed:
16
2
2021
medline:
11
8
2021
entrez:
15
2
2021
Statut:
ppublish
Résumé
The replicative polymerase δ (Polδ), consisting of four subunits, plays a pivotal role in chromosomal replication. Pold4, the smallest subunit of Polδ, is believed to contribute to the regulation of replication by facilitating repair in response to DNA damage. However, that contribution has not been fully elucidated. We here show that Pold4 contributes to the suppression of gene conversion in immunoglobulin-variable (IgV) gene diversification in the chicken DT40 lymphocyte cell line, where gene conversion diversifies the IgV gene through intragenic homologous recombination (HR) between diverged pseudo-V segments. IgV gene conversion is initiated by activation-induced cytidine deaminase-mediated uracil formation in the IgV gene, which in turn converts into an abasic site, leading to replication arrest. POLD4
Identifiants
pubmed: 33588156
pii: S1568-7864(21)00012-4
doi: 10.1016/j.dnarep.2021.103056
pii:
doi:
Substances chimiques
Immunoglobulin Variable Region
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
103056Informations de copyright
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