Structural basis of Stu2 recruitment to yeast kinetochores.
Ndc80 complex
S. cerevisiae
Stu2
cell biology
kinetochore
molecular biophysics
structural biology
structure
tension
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
16 02 2021
16 02 2021
Historique:
received:
02
12
2020
accepted:
15
02
2021
pubmed:
17
2
2021
medline:
22
1
2022
entrez:
16
2
2021
Statut:
epublish
Résumé
Chromosome segregation during cell division requires engagement of kinetochores of sister chromatids with microtubules emanating from opposite poles. As the corresponding microtubules shorten, these 'bioriented' sister kinetochores experience tension-dependent stabilization of microtubule attachments. The yeast XMAP215 family member and microtubule polymerase, Stu2, associates with kinetochores and contributes to tension-dependent stabilization in vitro. We show here that a C-terminal segment of Stu2 binds the four-way junction of the Ndc80 complex (Ndc80c) and that residues conserved both in yeast Stu2 orthologs and in their metazoan counterparts make specific contacts with Ndc80 and Spc24. Mutations that perturb this interaction prevent association of Stu2 with kinetochores, impair cell viability, produce biorientation defects, and delay cell cycle progression. Ectopic tethering of the mutant Stu2 species to the Ndc80c junction restores wild-type function in vivo. These findings show that the role of Stu2 in tension-sensing depends on its association with kinetochores by binding with Ndc80c.
Identifiants
pubmed: 33591274
doi: 10.7554/eLife.65389
pii: 65389
pmc: PMC7909949
doi:
pii:
Substances chimiques
Microtubule-Associated Proteins
0
NDC80 protein, S cerevisiae
0
Nuclear Proteins
0
STU2 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM124165
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007464
Pays : United States
Informations de copyright
© 2021, Zahm et al.
Déclaration de conflit d'intérêts
JZ, MS, JC, SH, MM No competing interests declared
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