A Balance Between Capture and Release: How Nanochaperones Regulate Refolding of Thermally Denatured Proteins.
controlled release
molecular chaperones
nanochaperones
protein refolding
self-assembly
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
03 05 2021
03 05 2021
Historique:
received:
30
01
2021
pubmed:
18
2
2021
medline:
10
8
2021
entrez:
17
2
2021
Statut:
ppublish
Résumé
Nanochaperones have been designed and used for regulating the (re)folding of proteins, treating protein misfolding-related diseases, and, more recently, in drug delivery. Despite various successes, a complete understanding of the working mechanisms remains elusive, which represents a challenge for the realization of their full potential. Here, we thoroughly investigated the functioning of differently charged nanochaperones that regulate the refolding of thermally denatured lysozyme. We found that the balance between the capture and release of lysozyme clients that are controlled by nanochaperones plays a key role in regulating refolding. More importantly, the findings could be applied to other enzymes with various physicochemical properties. On the basis of these results, we could recover the activity of enzymes with high efficiency either after 20 days of storage at 40 °C or heating at high temperatures for 30-60 min. Our results provide important new design strategies for nanochaperone systems to improve their properties and expand their applications.
Identifiants
pubmed: 33595165
doi: 10.1002/anie.202101462
doi:
Substances chimiques
Molecular Chaperones
0
Muramidase
EC 3.2.1.17
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10865-10870Informations de copyright
© 2021 Wiley-VCH GmbH.
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