A Reference Genome Assembly of American Bison, Bison bison bison.


Journal

The Journal of heredity
ISSN: 1465-7333
Titre abrégé: J Hered
Pays: United States
ID NLM: 0375373

Informations de publication

Date de publication:
29 03 2021
Historique:
received: 21 11 2020
accepted: 13 01 2021
pubmed: 18 2 2021
medline: 21 10 2021
entrez: 17 2 2021
Statut: ppublish

Résumé

Bison are an icon of the American West and an ecologically, commercially, and culturally important species. Despite numbering in the hundreds of thousands today, conservation concerns remain for the species, including the impact on genetic diversity of a severe bottleneck around the turn of the 20th century and genetic introgression from domestic cattle. Genetic diversity and admixture are best evaluated at genome-wide scale, for which a high-quality reference is necessary. Here, we use trio binning of long reads from a bison-Simmental cattle (Bos taurus taurus) male F1 hybrid to sequence and assemble the genome of the American plains bison (Bison bison bison). The male haplotype genome is chromosome-scale, with a total length of 2.65 Gb across 775 scaffolds (839 contigs) and a scaffold N50 of 87.8 Mb. Our bison genome is ~13× more contiguous overall and ~3400× more contiguous at the contig level than the current bison reference genome. The bison genome sequence presented here (ARS-UCSC_bison1.0) will enable new research into the evolutionary history of this iconic megafauna species and provide a new tool for the management of bison populations in federal and commercial herds.

Identifiants

pubmed: 33595645
pii: 6141414
doi: 10.1093/jhered/esab003
pmc: PMC8006816
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

174-183

Subventions

Organisme : NHGRI NIH HHS
ID : T32 HG008345
Pays : United States

Informations de copyright

© The American Genetic Association. 2021.

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Auteurs

Jonas Oppenheimer (J)

Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.

Benjamin D Rosen (BD)

USDA, ARS, Animal Genomics and Improvement Laboratory, Beltsville, MD, USA.

Michael P Heaton (MP)

USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA.

Brian L Vander Ley (BL)

Great Plains Veterinary Educational Center, University of Nebraska-Lincoln, Lincoln, NE, USA.

Wade R Shafer (WR)

American Simmental Association, Bozeman, MT, USA.

Fred T Schuetze (FT)

Simmentals of Texas, Granbury, TE, USA.

Brad Stroud (B)

Stroud Veterinary Embryo Services, Weatherford, TE, USA.

Larry A Kuehn (LA)

USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA.

Jennifer C McClure (JC)

USDA, ARS, U.S. Dairy Forage Research Center, Madison, WI, USA.

Jennifer P Barfield (JP)

College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.

Harvey D Blackburn (HD)

USDA, ARS, National Animal Germplasm Program, Fort Collins, CO, USA.

Theodore S Kalbfleisch (TS)

Gluck Equine Research Center, University of Kentucky, Lexington, KY, USA.

Derek M Bickhart (DM)

USDA, ARS, U.S. Dairy Forage Research Center, Madison, WI, USA.

Kimberly M Davenport (KM)

Department of Animal, Veterinary, and Food Science, University of Idaho, Moscow, ID, USA.

Kristen L Kuhn (KL)

USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA.

Richard E Green (RE)

Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.

Beth Shapiro (B)

Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, USA.
Howard Hughes Medical Institute, University of California Santa Cruz, Santa Cruz, CA, USA.

Timothy P L Smith (TPL)

USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE, USA.

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