Rat and human cytomegalovirus ORF116 encodes a virion envelope glycoprotein required for infectivity.
Cytomegalovirus
Glycoproteins
Viral entry
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
25
09
2020
revised:
22
12
2020
accepted:
23
12
2020
pubmed:
19
2
2021
medline:
29
12
2021
entrez:
18
2
2021
Statut:
ppublish
Résumé
Herpesviruses encode multiple glycoproteins required for different stages of viral attachment, fusion, and envelopment. The protein encoded by the human cytomegalovirus (HCMV) open reading frame UL116 forms a stable complex with glycoprotein H that is incorporated into virions. However, the function of this complex remains unknown. Herein, we characterize R116, the rat CMV (RCMV) putative homolog of UL116. Two R116 transcripts were identified in fibroblasts with three proteins expressed with molecular weights of 42, 58, and 82 kDa. R116 is N-glycosylated, expressed with late viral gene kinetics, and is incorporated into the virion envelope. RCMV lacking R116 failed to result in productive infection of fibroblasts and siRNA knockdown of R116 substantially reduced RCMV infectivity. Complementation in trans of an R116-deficient virus restored ability of the virus to infect fibroblasts. Finally, UL116 knockdown also decreased HCMV infectivity indicating that R116 and UL116 both contribute to viral infectivity.
Identifiants
pubmed: 33601113
pii: S0042-6822(20)30261-0
doi: 10.1016/j.virol.2020.12.014
pmc: PMC8019331
mid: NIHMS1674192
pii:
doi:
Substances chimiques
RNA, Double-Stranded
0
Viral Envelope Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
23-33Subventions
Organisme : NIAID NIH HHS
ID : F31 AI145193
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116633
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI116633
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007472
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
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