The role of long intergenic non-coding RNA for kinase activation (LINK-A) as an oncogene in non-small cell lung carcinoma.
A549 Cells
Apoptosis
/ genetics
Carcinogenesis
/ genetics
Carcinoma, Non-Small-Cell Lung
/ genetics
Cell Line, Tumor
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Cell Survival
/ genetics
Down-Regulation
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Humans
Lung Neoplasms
/ genetics
Oncogenes
/ genetics
RNA, Long Noncoding
/ genetics
RNA, Small Interfering
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 02 2021
18 02 2021
Historique:
received:
08
04
2020
accepted:
20
01
2021
entrez:
19
2
2021
pubmed:
20
2
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The oncogenic role of long intergenic non-coding RNA for kinase activation (LINK-A) has been appraised in triple-negative breast cancer. However, the molecular function of LINK-A is still unclear in most cancers including lung cancer. The present study aimed to evaluate the impact of down-regulation of LINK-A in A549 and Calu-3 cell lines as cellular models of non-small cell lung carcinoma (NSCLC). We used the RNA interference system to knock down LINK-A. LINK-A expression was significantly reduced by siRNA transfection in A549 and Calu-3 cell lines. LINK-A down-regulation significantly reduced cell viability, colony-forming ability and cell migration, as measured by MTT, colony formation and invasion assays. Finally, cell cycle analysis and Annexin-V/7AAD staining indicated that apoptosis was influenced by LINK-A silencing. Taken together, LINK-A can be proposed as an oncogene in NSCLC.
Identifiants
pubmed: 33602983
doi: 10.1038/s41598-021-82892-z
pii: 10.1038/s41598-021-82892-z
pmc: PMC7892821
doi:
Substances chimiques
LINK-A long non-coding RNA, human
0
RNA, Long Noncoding
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4210Références
J Biol Chem. 2002 Dec 6;277(49):47014-21
pubmed: 12354771
Anatol J Cardiol. 2018 Aug;20(2):100-109
pubmed: 30088484
Lung. 2016 Aug;194(4):637-46
pubmed: 27166634
Oncol Lett. 2019 Sep;18(3):3049-3057
pubmed: 31404323
Immunol Lett. 2013 Sep-Oct;155(1-2):36-9
pubmed: 24095849
Breast Cancer Res. 2018 Oct 1;20(1):117
pubmed: 30285805
Med Sci Monit. 2019 Mar 26;25:2221-2227
pubmed: 30912527
Cancer Res. 2011 Jan 1;71(1):3-7
pubmed: 21199792
Lancet. 2017 Jan 21;389(10066):299-311
pubmed: 27574741
Int J Mol Sci. 2012;13(2):1951-2011
pubmed: 22408433
PET Clin. 2018 Jan;13(1):1-10
pubmed: 29157380
J Clin Oncol. 2012 Apr 1;30(10):1137-9
pubmed: 22355057
Exp Mol Pathol. 2020 Jun;114:104411
pubmed: 32112788
Oncol Rep. 2017 Sep;38(3):1525-1532
pubmed: 28714007
Biomed Res Int. 2013;2013:746858
pubmed: 24171172
BMC Cancer. 2019 Mar 12;19(1):222
pubmed: 30866866
Database (Oxford). 2013 Oct 31;2013:bat074
pubmed: 24178989
Cancer Res. 2002 Jun 15;62(12):3373-6
pubmed: 12067977
Oncologist. 2004;9 Suppl 1:2-10
pubmed: 15178810
Br J Cancer. 2004 Aug 31;91(5):954-8
pubmed: 15328513
Curr Opin Genet Dev. 2017 Oct;46:170-178
pubmed: 28843809
Blood. 2008 Apr 1;111(7):3322-30
pubmed: 18362212
Hum Antibodies. 2020;28(3):227-232
pubmed: 32333582
Cancer Res. 2003 Jun 15;63(12):3403-12
pubmed: 12810677
Hepatology. 2011 Sep 2;54(3):910-9
pubmed: 21674552
Nat Cell Biol. 2016 Feb;18(2):213-24
pubmed: 26751287
Nat Genet. 2016 Jan;48(1):44-52
pubmed: 26595768
Breast Cancer Res Treat. 2013 Aug;140(3):505-17
pubmed: 23925655
Acta Pharm Sin B. 2015 Sep;5(5):378-89
pubmed: 26579469
Biochem J. 2000 Mar 15;346 Pt 3:603-10
pubmed: 10698685
Oncotarget. 2016 Jun 21;7(25):37773-37789
pubmed: 27191981
Br J Nurs. 2013 Sep 12-25;22(17):S15-6, S18, S20-2
pubmed: 24067269
Nat Cell Biol. 2017 Mar;19(3):238-251
pubmed: 28218907
Cancer Res. 2003 Oct 15;63(20):6651-7
pubmed: 14583458
Int J Oncol. 2012 Oct;41(4):1337-46
pubmed: 22825629
Oncol Rep. 2011 Apr;25(4):929-35
pubmed: 21308352
CA Cancer J Clin. 2018 Nov;68(6):394-424
pubmed: 30207593
J Thorac Oncol. 2009 Sep;4(9):1094-103
pubmed: 19687765
Gastric Cancer. 2018 Jul;21(4):632-642
pubmed: 29204860
Cell Death Dis. 2019 Feb 21;10(3):177
pubmed: 30792387
Blood. 2002 Nov 15;100(10):3767-75
pubmed: 12393646
Cell. 2008 Apr 4;133(1):66-77
pubmed: 18394990
J Cell Physiol. 2012 Feb;227(2):514-24
pubmed: 21503885
Oncogene. 2008 Oct 27;27(50):6398-406
pubmed: 18955968
Mol Cancer. 2011 Apr 13;10:38
pubmed: 21489289