Automated reanalysis, a novel way to diagnose an ultra-rare condition: Fibronectin-1-related spondylometaphyseal dysplasia (SMD-FN1).


Journal

Clinical dysmorphology
ISSN: 1473-5717
Titre abrégé: Clin Dysmorphol
Pays: England
ID NLM: 9207893

Informations de publication

Date de publication:
01 Jul 2021
Historique:
pubmed: 20 2 2021
medline: 24 11 2021
entrez: 19 2 2021
Statut: ppublish

Résumé

We report a further case of spondylometaphyseal dysplasia - corner fracture type due to the fibronectin-1 gene (SMD-FN1) in a child originally thought to have metaphyseal chondrodysplasia-Brussels type (MCD Brussels). We highlight phenotypic differences with the SMD-FN1 published reports. This case is unique in terms of the method of molecular confirmation. Findings from the 100 000 Genomes Project were originally negative (in both tier 1 and 2); however, subsequent reanalysis, initiated by an automated search for new gene-disease associations in PanelApp, highlighted a candidate diagnostic variant. Our child had short stature, facial dysmorphism, spondylometaphyseal dysplasia and corner fractures and a heterozygous de novo missense variant in FN1 (c.675C>G p.(Cys225Trp), which was likely pathogenic. The variant matched the clinical and radiological features and a diagnosis of SMD-FN1 was confirmed. We explore the diagnostic journey of this patient, compare her findings with the previous 15 patients reported with SMD-FN1 and discuss the diagnostic utility of automated reanalysis. We consider differences and similarities between MCD Brussels and SMD-FN1, by reviewing literature on both conditions and assess whether they are in fact the same disorder.

Identifiants

pubmed: 33605604
doi: 10.1097/MCD.0000000000000369
pii: 00019605-202107000-00009
doi:

Substances chimiques

FN1 protein, human 0
Fibronectins 0

Types de publication

Case Reports Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

154-158

Informations de copyright

Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.

Références

Cadoff EB, Sheffer R, Wientroub S, Ovadia D, Meiner V, Schwarzbauer JE (2018). Mechanistic insights into the cellular effects of a novel FN1 variant associated with a spondylometaphyseal dysplasia. Clin Genet. 94:429–437.
Costantini A, Valta H, Baratang NV, Yap P, Bertola DR, Yamamoto GL, et al. (2019). Novel fibronectin mutations and expansion of the phenotype in spondylometaphyseal dysplasia with “corner fractures”. Bone. 121:163–171.
Deignan JL, Chung WK, Kearney HM, Monaghan KG, Rehder CW, Chao EC (2019). Points to consider in the reevaluation and reanalysis of genomic test results: a statement of the American College of Medical Genetics and Genomics (ACMG). Genet Med. 21:1267–1270.
Lee CS, Fu H, Baratang N, Rousseau J, Kumra H, Sutton VR, et al.; Baylor-Hopkins Center for Mendelian Genomics (2017). Mutations in fibronectin cause a subtype of spondylometaphyseal dysplasia with “corner fractures”. Am J Hum Genet. 101:815–823.
Mievis C, Claus D, Clapuyt P, Nyssen-Behets C, Gosseye S, Malvaux P, Verellen-Dumoulin C (1996). A new familial short stature syndrome: Brussels type. Clin Dysmorphol. 5:9–16.
OMIM®. (2019). Online Mendelian Inheritance in Man, OMIM®. An Online Catalog of Human Genes and Genetic Disorders. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD). World Wide Web URL: https://omim.org/ [Accessed 30 July 2020].
Richards S, Aziz N, Bale S, Bick D, Das S, Gastier-Foster J, et al.; ACMG Laboratory Quality Assurance Committee (2015). Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med. 17:405–424.
Singh P, Carraher C, Schwarzbauer JE (2010). Assembly of fibronectin extracellular matrix. Annu Rev Cell Dev Biol. 26:397–419.
Turnbull C, Scott RH, Thomas E, Jones L, Murugaesu N, Pretty FB, et al. (2018). The 100 000 genomes project: bringing whole genome sequencing to the NHS. BMJ. 361:k1687.

Auteurs

Ataf H Sabir (AH)

Clinical Genetics Registrar, Guy's and St Thomas' NHS Foundation Trust (London), Birmingham Women's and Children's Hospital NHS Trust & Birmingham Health Partners.

Juhi Singhal (J)

Medical Student, University of Birmingham, Birmingham.

Jessica Man (J)

Medical Student, University of Birmingham, Birmingham.

Nana Ekuntan Mensah (NE)

Trainee Clinical Bioinformatician, Guy's and St Thomas' NHS Foundation Trust, London.

Joo Wook Ahn (JW)

East England GLH Bioinformatics Lead, Cambridge University Hospitals NHS FT, Addenbrooke's Hospital, Cambridge.

Moira S Cheung (MS)

Consultant Paediatric Endocrinologist.

Melita Irving (M)

Consultant Clinical Geneticist, Guy's and St Thomas' NHS Foundation Trust, London, UK.

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