Estrogen deficiency affects tooth formation and gene expression in the odontogenic region of female rats.
Estrogen
Gene expression
Odontogenesis
Tooth morphology
Journal
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft
ISSN: 1618-0402
Titre abrégé: Ann Anat
Pays: Germany
ID NLM: 100963897
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
06
11
2020
revised:
13
01
2021
accepted:
25
01
2021
pubmed:
20
2
2021
medline:
3
11
2021
entrez:
19
2
2021
Statut:
ppublish
Résumé
There is some evidence that estrogen regulates the expression of several genes in different cells, including dental cells. Therefore, the aim of this study was to investigate the role of estrogen deficiency during tooth development regarding tooth structure morphology and its impact on the expression of odontogenesis-related genes. A total of 40 female Wistar rats was divided into OVX (estrogen deficiency) and Sham (control) groups. Bilateral ovariectomy was performed in the OVX group, while Sham surgery was performed in the control group at the age of 21 days. At an age of 56 days, 16 rats were euthanized for gene expression analyses of Bmp4, Smad6, Tgfb1 and Runx2. At the age of 63 days, the remaining rats were euthanized for histological and morphometric analyses of teeth. The mandibles of the rats were submitted to μCT analysis. Tooth structures (enamel, dentin and dental pulp) were analyzed. T test was used to compare the mean differences between groups (p<0.05). In the μCT analysis, enamel and dentin thickness were significantly increased in the control group (p<0.0001). Pulp dimensions were significantly larger in the OVX group (p<0.0001). A reduction of tooth structures in the OVX group was confirmed in HE staining. Smad6 was differentially expressed in the OVX group (p=0.04). Estrogen deficiency affects gene expression in the odontogenic region and tooth structure morphology.
Sections du résumé
BACKGROUND
BACKGROUND
There is some evidence that estrogen regulates the expression of several genes in different cells, including dental cells. Therefore, the aim of this study was to investigate the role of estrogen deficiency during tooth development regarding tooth structure morphology and its impact on the expression of odontogenesis-related genes.
METHODS
METHODS
A total of 40 female Wistar rats was divided into OVX (estrogen deficiency) and Sham (control) groups. Bilateral ovariectomy was performed in the OVX group, while Sham surgery was performed in the control group at the age of 21 days. At an age of 56 days, 16 rats were euthanized for gene expression analyses of Bmp4, Smad6, Tgfb1 and Runx2. At the age of 63 days, the remaining rats were euthanized for histological and morphometric analyses of teeth. The mandibles of the rats were submitted to μCT analysis. Tooth structures (enamel, dentin and dental pulp) were analyzed. T test was used to compare the mean differences between groups (p<0.05).
RESULTS
RESULTS
In the μCT analysis, enamel and dentin thickness were significantly increased in the control group (p<0.0001). Pulp dimensions were significantly larger in the OVX group (p<0.0001). A reduction of tooth structures in the OVX group was confirmed in HE staining. Smad6 was differentially expressed in the OVX group (p=0.04).
CONCLUSION
CONCLUSIONS
Estrogen deficiency affects gene expression in the odontogenic region and tooth structure morphology.
Identifiants
pubmed: 33607226
pii: S0940-9602(21)00028-5
doi: 10.1016/j.aanat.2021.151702
pii:
doi:
Substances chimiques
Estrogens
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
151702Informations de copyright
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