Tryptophan as a Safe Compound in Topical Ophthalmic Medications:
Cell culture
Tryptophan
conjunctival epithelium
corneal epithelium
rabbit
Journal
Ocular immunology and inflammation
ISSN: 1744-5078
Titre abrégé: Ocul Immunol Inflamm
Pays: England
ID NLM: 9312169
Informations de publication
Date de publication:
19 May 2022
19 May 2022
Historique:
pubmed:
23
2
2021
medline:
2
9
2022
entrez:
22
2
2021
Statut:
ppublish
Résumé
To evaluate the effects of tryptophan (TRP) on normal human corneal and conjunctival epithelium in vitro and the re-epithelization of corneal erosion in rabbits. Corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) cultures were used. The cellular metabolism, viability, secretion of IL-1β, IL-6, IL-10, cytoskeleton organization, transwell migration were determined. Cells were incubated in the presence of TRP at 1-100 μM. After corneal de-epithelization rabbits received TRP drops (100 μM), 5 times a day. TRP increased conjunctival epithelium metabolism at 50 μM and increased the viability of corneal epithelium at 100 μM. TRP (10 μM) enhanced the production of IL-6 by the corneal epithelium and had no effect on IL-1β and IL-10. TRP had no influence on the cellular cytoskeleton but induced a significant pseudopodia projection in both epithelia. TRP did not influence corneal re-epithelization in vivo. TRP was not toxic for corneal and conjunctival epithelia.
Sections du résumé
BACKGROUND
UNASSIGNED
To evaluate the effects of tryptophan (TRP) on normal human corneal and conjunctival epithelium in vitro and the re-epithelization of corneal erosion in rabbits.
MATERIALS AND METHODS
UNASSIGNED
Corneal epithelial cell (10.014 pRSV-T) and conjunctival epithelial cell (HC0597) cultures were used. The cellular metabolism, viability, secretion of IL-1β, IL-6, IL-10, cytoskeleton organization, transwell migration were determined. Cells were incubated in the presence of TRP at 1-100 μM. After corneal de-epithelization rabbits received TRP drops (100 μM), 5 times a day.
RESULTS
UNASSIGNED
TRP increased conjunctival epithelium metabolism at 50 μM and increased the viability of corneal epithelium at 100 μM. TRP (10 μM) enhanced the production of IL-6 by the corneal epithelium and had no effect on IL-1β and IL-10.
CONCLUSIONS
UNASSIGNED
TRP had no influence on the cellular cytoskeleton but induced a significant pseudopodia projection in both epithelia. TRP did not influence corneal re-epithelization in vivo. TRP was not toxic for corneal and conjunctival epithelia.
Identifiants
pubmed: 33616466
doi: 10.1080/09273948.2020.1856883
doi:
Substances chimiques
Interleukin-6
0
Interleukin-10
130068-27-8
Tryptophan
8DUH1N11BX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM