Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods.
Adipose tissue-derived stem cells
Culture conditions
Differentiation protocols
Isolation procedure
Stromal vascular fraction
Journal
Cell and tissue banking
ISSN: 1573-6814
Titre abrégé: Cell Tissue Bank
Pays: Netherlands
ID NLM: 100965121
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
17
11
2020
accepted:
01
02
2021
pubmed:
23
2
2021
medline:
22
2
2022
entrez:
22
2
2021
Statut:
ppublish
Résumé
Adipose tissue-derived stem cells (ADSCs) are an available source of mesenchymal stem cells with the appropriate capacity to in vitro survive, propagate, and differentiate into cells from three lineages of ectoderm, mesoderm, and endoderm. The biological features of ADSCs depend on the donor physiology and health status, isolation procedure, culture conditions, and differentiation protocols used. Adipose tissue samples are provided by surgery and lipoaspiration-based methods and subjected to various mechanical and chemical digestion techniques to finally generate a heterogeneous mixture named stromal vascular fraction (SVF). ADSCs are purified through varied cell populations that exist within SVF and cultured under standard conditions to give rise to a highly rich resource of stem cells directly applied in the clinic or differentiated into a wide range of cells. The development and optimization of conventional isolation, expansion, and differentiation methods seem noteworthy to preserve the desirable biological functions of ADSCs in pre-clinical and clinical investigations.
Identifiants
pubmed: 33616792
doi: 10.1007/s10561-021-09905-z
pii: 10.1007/s10561-021-09905-z
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-16Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.
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