Identification, isolation and analysis of human gut-associated lymphoid tissues.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
21
06
2020
accepted:
09
12
2020
pubmed:
24
2
2021
medline:
5
5
2021
entrez:
23
2
2021
Statut:
ppublish
Résumé
Gut-associated lymphoid tissues (GALTs) comprise key intestinal immune inductive sites, including the Peyer's patches of the small intestine and different types of isolated lymphoid follicle (ILF) found along the length of the gut. Our understanding of human GALT is limited due to a lack of protocols for their isolation. Here we describe a technique that, uniquely among intestinal cell isolation protocols, allows identification and isolation of all human GALT, as well as GALT-free intestinal lamina propria (LP). The technique involves the mechanical separation of intestinal mucosa from the submucosa, allowing the identification and isolation of submucosal ILF (SM-ILF), LP-embedded mucosal ILF (M-ILF) and LP free of contaminating lymphoid tissue. Individual SM-ILF, M-ILF and Peyer's patch follicles can be subsequently digested for downstream cellular and molecular characterization. The technique, which takes 4-10 h, will be useful for researchers interested in intestinal immune development and function in health and disease.
Identifiants
pubmed: 33619391
doi: 10.1038/s41596-020-00482-1
pii: 10.1038/s41596-020-00482-1
doi:
Substances chimiques
Leukocyte Common Antigens
EC 3.1.3.48
Banques de données
figshare
['10.6084/m9.figshare.13060592']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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