Genome-scale deconvolution of RNA structure ensembles.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
17
09
2020
accepted:
19
01
2021
pubmed:
24
2
2021
medline:
23
3
2021
entrez:
23
2
2021
Statut:
ppublish
Résumé
RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3' untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.
Identifiants
pubmed: 33619392
doi: 10.1038/s41592-021-01075-w
pii: 10.1038/s41592-021-01075-w
doi:
Substances chimiques
3' Untranslated Regions
0
RNA, Viral
0
Sulfuric Acid Esters
0
dimethyl sulfate
JW5CW40Z50
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
249-252Références
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