First Observation of HbM-Saskatoon at the Origin of Neonatal Cyanosis in a Tunisian Baby.


Journal

Journal of pediatric hematology/oncology
ISSN: 1536-3678
Titre abrégé: J Pediatr Hematol Oncol
Pays: United States
ID NLM: 9505928

Informations de publication

Date de publication:
01 11 2021
Historique:
received: 11 08 2020
accepted: 08 01 2021
pubmed: 25 2 2021
medline: 6 1 2022
entrez: 24 2 2021
Statut: ppublish

Résumé

Several causes are known to be at the origin of neonatal cyanosis among them methemoglobinemia is by inheritance of an hemoglobin (Hb) M variant. This is a rare condition never been reported in Tunisia so far. Here, we report a Tunisian newborn with refractory cyanosis since birth. As cardiac and respiratory diseases were ruled out, methemoglobinemia was suspected. Hematological parameters, concentration of methemoglobin, capillary electrophoresis, and amplification sequencing of the HBB gene were performed. Computational analysis was achieved by different in silico tools to investigate the mutation effect. The diagnosis was established by a raised MetHb, confirmed by the presence HbM-Saskatoon [Beta63 (E7) His>Tyr] by capillary electrophoresis and molecular analysis. The identified mutation occurred as a de novo mutation. In silico analysis confirmed the pathogenicity of the mutation. To our knowledge, this is the first time that this mutation has been reported in the Tunisian population. In view of its low incidence rate, clinicians might misdiagnose cyanosis caused by HbM, which can lead to inappropriate treatment and clinical complications. An up-to-date literature review of HbM disease is presented in this study.

Identifiants

pubmed: 33625083
doi: 10.1097/MPH.0000000000002096
pii: 00043426-202111000-00008
doi:

Substances chimiques

Hemoglobins, Abnormal 0
hemoglobin Saskatoon 69670-54-8
Hemoglobin M 9083-28-7

Types de publication

Case Reports Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1055-e1058

Informations de copyright

Copyright © 2021 Wolters Kluwer Health, Inc. All rights reserved.

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

Références

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Auteurs

Emna Bouatrous (E)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.

Sonia Nouira (S)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.
Molecular Biology Cell and Biotechnology Department, Higher Institute of Biotechnology of Monastir, University of Monastir, Monastir, Tunisia.

Monia Ben Khaled (M)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.
Pediatric Immuno-Hematology Unit, Bone Marrow Transplantation Center, Tunis.

Monia Ouederni (M)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.
Pediatric Immuno-Hematology Unit, Bone Marrow Transplantation Center, Tunis.

Salem Abbes (S)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.

Samia Menif (S)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.

Houyem Ouragini (H)

Laboratory of Molecular and Cellular Hematology, LR16IPT07, Pasteur Institute of Tunis, University of Tunis El Manar.

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