Antibiotic Resistance in Pseudomonas spp. Through the Urban Water Cycle.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
26
05
2020
accepted:
07
02
2021
pubmed:
25
2
2021
medline:
15
5
2021
entrez:
24
2
2021
Statut:
ppublish
Résumé
Selection and dissemination of resistant bacteria and antibiotic resistance genes (ARGs) require a deeper understanding since antibiotics are permanently released to the environment. The objective of this paper was to evaluate the phenotypic resistance of 499 isolates of Pseudomonas spp. from urban water sources, and the prevalence of 20 ARGs within those isolates. Resistance to penicillins, cephalosporins, carbapenems, quinolones, macrolides, and tetracyclines was mainly observed in the hospital effluent, municipal wastewater and river water downstream the city. Resistant strains were frequently identified as P. aeruginosa and P. putida. P. aeruginosa isolates were mostly resistant to cefepime, ceftazidime, imipenem, and gentamycin, while P. putida strains were especially resistant to piperacillin-tazobactam. ARGs such as bla
Identifiants
pubmed: 33625570
doi: 10.1007/s00284-021-02389-w
pii: 10.1007/s00284-021-02389-w
doi:
Substances chimiques
Anti-Bacterial Agents
0
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1227-1237Subventions
Organisme : EEA Grants
ID : 471 3499/20.05.2015
Organisme : Ministry of Research and Innovation Romania
ID : 22PFE / 2018
Organisme : Ministry of Research and Innovation Romania
ID : 25 N/2019 - BIOSERV
Références
Meyer E, Gastmeier P, Deja M, Schwab F (2013) Antibiotic consumption and resistance: data from Europe and Germany. Int J Med Microbiol 303:388–395
pubmed: 23727396
doi: 10.1016/j.ijmm.2013.04.004
Chao Y, Ma L, Yang Y, Ju F, Zhang X-X, Wu W-M, Zhang T (2013) Metagenomic analysis reveals significant changes of microbial compositions and protective functions during drinking water treatment. Sci Rep. https://doi.org/10.1038/srep03550
pubmed: 24352003
pmcid: 6506563
doi: 10.1038/srep03550
Gruber I, Heudorf U, Werner G, Pfeifer Y, Imirzalioglu C, Ackermann H, Brandt C, Besier S, Wichelhaus TA (2013) Multidrug-resistant bacteria in geriatric clinics, nursing homes, and ambulant care-prevalence and risk factors. Int J Med Microbiol 303:405–409
pubmed: 23770266
doi: 10.1016/j.ijmm.2013.05.002
Martins VV, Zanetti MOB, Pitondo-Silva A, Stehling EG (2014) Aquatic environments polluted with antibiotics and heavy metals: a human health hazard. Environ Sci Pollut Res 21:5873–5878
doi: 10.1007/s11356-014-2509-4
Nõlvak H, Truu M, Kanger K, Tampere M, Espenberg M, Loit E, Raave H, Truu J (2016) Inorganic and organic fertilizers impact the abundance and proportion of antibiotic resistance and integron-integrase genes in agricultural grassland soil. Sci Total Environ 562:678–689
pubmed: 27115621
doi: 10.1016/j.scitotenv.2016.04.035
Cai L, Ju F, Zhang T (2014) Tracking human sewage microbiome in a municipal wastewater treatment plant. Appl Microbiol Biotechnol 98:3317–3326
pubmed: 24305737
doi: 10.1007/s00253-013-5402-z
Szekeres E, Baricz A, Chiriac CM, Farkas A, Opris O, Soran ML, Andrei AS, Rudi K, Balcázar JL, Dragos N, Coman C (2017) Abundance of antibiotics, antibiotic resistance genes and bacterial community composition in wastewater effluents from different Romanian hospitals. Environ Pollut 225:304–315
pubmed: 28347610
doi: 10.1016/j.envpol.2017.01.054
Stalder T, Barraud O, Jove T, Casellas M, Gaschet M, Dagot C, Ploy M-C (2014) Quantitative and qualitative impact of hospital effluent on dissemination of the integron pool. ISME J 8:768–777
pubmed: 24152716
doi: 10.1038/ismej.2013.189
Borruso L, Harms K, Johnsen PJ, Nielsen KM, Brusetti L (2016) Distribution of class 1 integrons in a highly impacted catchment. Sci Total Environ 566–567:1588–1594
pubmed: 27312274
doi: 10.1016/j.scitotenv.2016.06.054
Li L-G, Yin X, Zhang T (2018) Tracking antibiotic resistance gene pollution from different sources using machine-learning classification. Microbiome 6:93
pubmed: 29793542
pmcid: 5966912
doi: 10.1186/s40168-018-0480-x
Nardelli M, Scalzo PM, Ramírez MS, Quiroga MP, Cassini MH, Centrón D (2012) Class 1 integrons in environments with different degrees of urbanization. PLoS ONE 6:7
Gillings MR, Gaze WH, Pruden A, Smalla K, Tiedje JM, Zhu YG (2015) Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution. ISME J 9(6):1269–1279
pubmed: 25500508
doi: 10.1038/ismej.2014.226
Peix A, Ramírez-Bahenac M-H, Velázquez E (2018) The current status on the taxonomy of Pseudomonas revisited: an update. Infect Genet Evol 57:106–116
pubmed: 29104095
doi: 10.1016/j.meegid.2017.10.026
Dantas RCC, Silva RTE, Ferreira ML, Goncalves IR, Araújo BF, Campos PA, Royer S, Batistão DWDF, Gontijo-Filho PP, Ribas RM (2017) Molecular epidemiological survey of bacteremia by multidrug resistant Pseudomonas aeruginosa: the relevance of intrinsic resistance mechanisms. PLoS ONE 12:e0176774
pubmed: 28481953
pmcid: 5421754
doi: 10.1371/journal.pone.0176774
Tsao LH, Hsin C-Y, Liu H-Y, Chuang H-C, Chen L-Y, Lee Y-J (2018) Risk factors for healthcare-associated infection caused by carbapenem-resistant Pseudomonas aeruginosa. J Microbiol Immunol Infect 51:359–366
pubmed: 28988663
doi: 10.1016/j.jmii.2017.08.015
Falup-Pecurariu O, Leibovitz E, Mercas A, Bleotu L, Zavarache C, Porat N, Dagan R, Greenberg D (2012) Pneumococcal acute otitis media in infants and children in central Romania, 2009–2011: microbiological characteristics and potential coverage by pneumococcal conjugate vaccines. Int J Infect Dis 17:e702–e706
doi: 10.1016/j.ijid.2013.02.002
Cucu A, Nica M, Ceaușu E, Cioran N (2014) Antimicrobial resistance profile in infectious disease hospital intensive care unit. Farmacia 62:767–776
Slavcovici A, Maier C, Radulescu A (2015) Antimicrobial resistance of ESKAPE-pathogens in culture-positive pneumonia. Farmacia 63:201–205
Farkas A, Bocoș B, Butiuc-Keul A (2016) Antibiotic resistance and intI1 carriage in waterborne Enterobacteriaceae. Water Air Soil Pollut 227:251. https://doi.org/10.1007/s11270-016-2944-6
doi: 10.1007/s11270-016-2944-6
Crăciunaş C, Butiuc-Keul A, Flonta M, Brad A, Sigarteu M (2010) Application of molecular techniques to the study of Pseudomonas aeruginosa clinical isolate in Cluj-Napoca, Romania. Ann Univ Oradea Biology 243–247
Dortet L, Flonta M, Boudehen Y-M, Creton E, Bernabeu S, Vogel A, Naasa T (2015) Dissemination of Carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa in Romania. Antimicrob Agents Chemother 59:7100–7103
pubmed: 26303798
pmcid: 4604391
doi: 10.1128/AAC.01512-15
Lixandru BE, Cotar AI, Straut M, Usein CR, Cristea D, Ciontea S, Tatu-Chitoiu D, Codita I, Rafila A, Nica M, Buzea M, Baicus A, Ghita MC, Nistor I, Tuchiluş C, Indreas M, Antohe F, Glasner C, Grundmann H, Jasir A, Damian M (2015) Carbapenemase-producing Klebsiella pneumoniae in Romania: a six-month survey. PLoS ONE. https://doi.org/10.1371/journal.pone.0143214
pubmed: 26599338
pmcid: 4658179
doi: 10.1371/journal.pone.0143214
Kittinger C, Lipp M, Baumert R, Folli B, Koraimann G, Toplitsch D, Liebmann A, Grisold AJ, Farnleitner AH, Kirschner A, Zarfel G (2016) Antibiotic resistance patterns of Pseudomonas spp. isolated from the river Danube. Front Microbiol. https://doi.org/10.3389/fmicb.2016.00586
pubmed: 27199920
pmcid: 4853796
doi: 10.3389/fmicb.2016.00586
https://www.numbeo.com/quality-of-life/country_result.jsp?country=Romania . Accessed 20.05.2020
Widmer F, Seidler RJ, Gillevet PM, Watrud LS, Di Giovanni GD (1998) A highly selective PCR protocol for detecting 16S rRNA genes of the genus Pseudomonas (sensu stricto) in environmental samples. Appl Environ Microbiol 64:2545–2553
pubmed: 9647828
pmcid: 106424
doi: 10.1128/AEM.64.7.2545-2553.1998
EUCAST (2014) Antimicrobial susceptibility testing. EUCAST disk diffusion method. European Committee on Antimicrobial Susceptibility Testing, Stockholm
Farkas A (2016) Antibiotic susceptibility testing by disc diffusion method. In: Coman C (ed) Methodological guide for monitoring antibiotics and antibiotic resistance in the environment. Accent Publisher, Cluj-Napoca, pp 253–276
EUCAST (2015) Breakpoint tables for interpretation of MICs and zone diameters. Version 5. European Committee on Antimicrobial Susceptibility Testing, Stockholm
Hammer Ø, Harper DAT, Ryan PD (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron 4(1):9
Vaz-Moreira I, Nunes OC, Manaia CM (2012) Diversity and antibiotic resistance in Pseudomonas spp. from drinking water. Sci Total Environ 426:366–374
pubmed: 22521167
doi: 10.1016/j.scitotenv.2012.03.046
González F, Araque M (2013) Association of transferable quinolone resistance determinant qnrB19 with extended-spectrum β-lactamases in Salmonella Give and Salmonella Heidelberg in Venezuela. J Microbiol. https://doi.org/10.1155/2013/628185
doi: 10.1155/2013/628185
Mokracka J, Koczura R, Kaznowski A (2012) Multiresistant Enterobacteriaceae with class 1 and class 2 integrons in a municipal wastewater treatment plant. Water Res 46:3353–3363
pubmed: 22507248
doi: 10.1016/j.watres.2012.03.037
Zaborina O, Holbrook C, Chen Y, Long J, Zaborin A, Morozova I, Fernandez H, Wang Y, Turner JR, Alverdy JC (2008) Structure-function aspects of PstS in multi-drug-resistant Pseudomonas aeruginosa. PLoS Pathog 4(2):e43. https://doi.org/10.1371/journal.ppat.0040043
pubmed: 18282104
pmcid: 2242829
doi: 10.1371/journal.ppat.0040043
Poole K (2005) Aminoglycoside resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother 49:479–487
pubmed: 15673721
pmcid: 547279
doi: 10.1128/AAC.49.2.479-487.2005
Poole K (2011) Pseudomonas aeruginosa: resistance to the max. Front Microbiol 2:65
pubmed: 21747788
pmcid: 3128976
doi: 10.3389/fmicb.2011.00065
CLSI (2015) Performance standards for antimicrobial susceptibility testing; twenty-fifth informational supplement M100–S25. Clinical and Laboratory Standards Institute, Wayne
EUCAST (2016) EUCAST Expert Rules Version 3.1. European Committee on Antimicrobial Susceptibility Testing, Stockholm
Farkas A, Crăciunaş C, Chiriac C, Szekeres E, Coman C, Butiuc-Keul A (2016) Exploring the role of coliform bacteria in class 1 integron carriage and biofilm formation during drinking water treatment. Microb Ecol 72(4):773–782
pubmed: 27079455
doi: 10.1007/s00248-016-0758-0
Figueira V, Serra EA, Vaz-Moreira I, Brandão TR, Manaia CM (2012) Comparison of ubiquitous antibiotic-resistant Enterobacteriaceae populations isolated from wastewaters, surface waters and drinking waters. J Water Health 10:1–10
pubmed: 22361697
doi: 10.2166/wh.2011.002
Koczura R, Mokracka J, Jabłońska L, Gozdecka E, Kubek M, Kazonowksi A (2012) Antimicrobial resistance of integron-harboring Escherichia coli isolates from clinical samples, wastewater treatment plant and river water. Sci Total Environ 414:680–685
pubmed: 22119028
doi: 10.1016/j.scitotenv.2011.10.036
Wellington EMH, Boxall ABA, Cross P, Feil EJ, Gaze WH, Hawkey PM, Johnson-Rollings AS, Jones DL, Lee NM, Otten W, Thomas CM, Williams AP (2013) The role of the natural environment in the emergence of antibiotic resistance in Gram-negative bacteria. Lancet Infect Dis 13:155–165
pubmed: 23347633
doi: 10.1016/S1473-3099(12)70317-1
Ben W, Wang J, Cao R, Yang M, Zhang Y, Qiang Z (2017) Distribution of antibiotic resistance in the effluents of ten municipal wastewater treatment plants in China and the effect of treatment processes. Chemosphere 172:392–398
pubmed: 28088530
doi: 10.1016/j.chemosphere.2017.01.041
Stokes HW, Gillings MR (2011) Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens. FEMS Microbiol Rev 35:790–819
pubmed: 21517914
doi: 10.1111/j.1574-6976.2011.00273.x
Lim CLL, Chuaa AQ, Teoa JQM, Caia Y, Leea W, Lay-Hoon Kwaa A (2018) Importance of control groups when delineating antibiotic use as a risk factor for carbapenem resistance, extreme-drug resistance, and pan-drug resistance in Acinetobacter baumannii and Pseudomonas aeruginosa: a systematic review and meta-analysis. Int J Infect Dis 76:48–57
pubmed: 29870795
doi: 10.1016/j.ijid.2018.05.017
WHO (2014) World Health Organization. Antimicrobial Resistance: Global report on surveillance. WHO Press, Geneva
Alexopoulos A, Voidarou C, Stefani C, Papadopoulos I, Vavias S, Tsiotsias A, Kalkani E, Charvalos E, Bezirtzoglou E (2006) Antibiotic resistance profiles and integrons in Enterobacteriaceae from the riverside of Evros-Ardas with respect to chemical and waste pollution. Microb Ecol Health Dis 18:170–176
Guo X, Xia R, Han N, Xu H (2011) Genetic diversity analyses of class 1 integrons and their associated antimicrobial resistance genes in Enterobacteriaceae strains recovered from aquatic habitats in China. Lett Appl Microbiol 52:667–675
pubmed: 21496063
doi: 10.1111/j.1472-765X.2011.03059.x
Cho CH, Lee SB (2018) Comparison of clinical characteristics and antibiotic susceptibility between Pseudomonas aeruginosa and P. putida keratitis at a tertiary referral center: a retrospective study. BMC Ophthalmol 18:204
pubmed: 30126384
pmcid: 6102849
doi: 10.1186/s12886-018-0882-3
Finklea JD, Hollaway R, Lowe K, Lee F, Le J, Jain R (2018) Ceftolozane/tazobactam sensitivity patterns in Pseudomonas aeruginosa isolates recovered from sputum of cystic fibrosis patients. Diagn Microbiol Infect Dis 92:75–77
pubmed: 29884565
doi: 10.1016/j.diagmicrobio.2018.05.002
European Commission (EC) (2000) Directive 200/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Off J EC LI 372(43):1e73