Chicken cGAS Senses Fowlpox Virus Infection and Regulates Macrophage Effector Functions.
Animals
Cell Line
Chickens
/ metabolism
DNA Viruses
/ genetics
DNA, Viral
/ genetics
Fowlpox
/ metabolism
Fowlpox virus
/ genetics
Histocompatibility Antigens Class II
/ metabolism
Interferon Type I
/ metabolism
Macrophages
/ metabolism
Membrane Proteins
/ metabolism
Nucleotides, Cyclic
/ metabolism
Signal Transduction
/ physiology
DNA
chicken
cyclic GMP-AMP synthase
fowlpox
macrophages
pattern recognition receptor
stimulator of interferon genes
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
10
2020
accepted:
16
12
2020
entrez:
26
2
2021
pubmed:
27
2
2021
medline:
23
6
2021
Statut:
epublish
Résumé
The anti-viral immune response is dependent on the ability of infected cells to sense foreign nucleic acids. In multiple species, the pattern recognition receptor (PRR) cyclic GMP-AMP synthase (cGAS) senses viral DNA as an essential component of the innate response. cGAS initiates a range of signaling outputs that are dependent on generation of the second messenger cGAMP that binds to the adaptor protein stimulator of interferon genes (STING). Here we show that in chicken macrophages, the cGAS/STING pathway is essential not only for the production of type-I interferons in response to intracellular DNA stimulation, but also for regulation of macrophage effector functions including the expression of MHC-II and co-stimulatory molecules. In the context of fowlpox, an avian DNA virus infection, the cGAS/STING pathway was found to be responsible for type-I interferon production and MHC-II transcription. The sensing of fowlpox virus DNA is therefore essential for mounting an anti-viral response in chicken cells and for regulation of a specific set of macrophage effector functions.
Identifiants
pubmed: 33633733
doi: 10.3389/fimmu.2020.613079
pmc: PMC7901977
doi:
Substances chimiques
DNA, Viral
0
Histocompatibility Antigens Class II
0
Interferon Type I
0
Membrane Proteins
0
Nucleotides, Cyclic
0
cyclic guanosine monophosphate-adenosine monophosphate
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
613079Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/G018545/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/H005323/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K002465/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S001336/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/E009956/1
Pays : United Kingdom
Informations de copyright
Copyright © 2021 Oliveira, Rodrigues, Guillory, Kut, Giotis, Skinner, Guabiraba, Bryant and Ferguson.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Microbes Infect. 2014 Dec;16(12):1002-12
pubmed: 25316508
Nat Rev Immunol. 2012 Jun 22;12(7):479-91
pubmed: 22728526
J Immunol. 2003 Oct 15;171(8):4320-8
pubmed: 14530357
Cell Rep. 2019 Oct 29;29(5):1236-1248.e7
pubmed: 31665636
Dev Comp Immunol. 2016 Jun;59:145-52
pubmed: 26828392
EMBO Rep. 2015 Feb;16(2):202-12
pubmed: 25572843
Dev Comp Immunol. 2018 Sep;86:156-170
pubmed: 29729283
Vet Res. 2016 Aug 05;47(1):75
pubmed: 27494935
Nat Cell Biol. 2017 Sep;19(9):1061-1070
pubmed: 28759028
Sci Signal. 2012 Mar 06;5(214):ra20
pubmed: 22394562
J Interferon Cytokine Res. 2004 Mar;24(3):179-84
pubmed: 15035851
Pharmacol Rev. 2015;67(2):462-504
pubmed: 25829385
J Virol. 2010 Jul;84(13):6549-63
pubmed: 20410285
Cell. 1979 Oct;18(2):375-90
pubmed: 227607
Cell Host Microbe. 2013 Oct 16;14(4):422-34
pubmed: 24139400
J Immunol. 2017 Nov 1;199(9):3222-3233
pubmed: 28939760
Immunity. 2006 Jan;24(1):93-103
pubmed: 16413926
J Exp Med. 2018 May 7;215(5):1287-1299
pubmed: 29622565
Avian Pathol. 2010 Oct;39(5):309-24
pubmed: 20954007
PLoS Pathog. 2019 Sep 20;15(9):e1007999
pubmed: 31539404
Curr Opin Immunol. 2017 Jun;46:23-29
pubmed: 28433952
Methods Mol Biol. 2016;1338:261-72
pubmed: 26443227
Nature. 2013 Jun 20;498(7454):380-4
pubmed: 23722158
J Virol. 2019 Feb 5;93(4):
pubmed: 30518647
Hum Mol Genet. 2009 Oct 15;18(R2):R130-6
pubmed: 19808788
Avian Pathol. 2019 Apr;48(2):87-90
pubmed: 30507248
J Immunol. 2019 Oct 1;203(7):1930-1942
pubmed: 31366714
J Gen Virol. 2013 Nov;94(Pt 11):2367-2392
pubmed: 23999164
Nat Commun. 2017 Sep 5;8(1):427
pubmed: 28874664
Int J Biol Macromol. 2020 Mar 1;146:497-507
pubmed: 31923489
Nature. 2005 Feb 24;433(7028):887-92
pubmed: 15711573
Nature. 2013 Nov 28;503(7477):530-4
pubmed: 24077100
Vet Pathol. 2010 Jan;47(1):177-80
pubmed: 20080499
Cell. 2017 Nov 16;171(5):1110-1124.e18
pubmed: 29033128
Science. 2013 Sep 20;341(6152):1390-4
pubmed: 23989956
Nucleic Acids Res. 1995 Jun 25;23(12):2137-46
pubmed: 7541908
J Virol. 2013 May;87(9):5041-52
pubmed: 23427153
EMBO J. 2019 Mar 1;38(5):
pubmed: 30696688
Nat Rev Drug Discov. 2018 Dec 28;17(1):3-5
pubmed: 29282375
Mol Cell. 1997 Dec;1(1):119-29
pubmed: 9659909
Clin Immunol. 2013 Jun;147(3):207-15
pubmed: 23419883
Biomedicines. 2020 Dec 19;8(12):
pubmed: 33352813