Integron activity accelerates the evolution of antibiotic resistance.
P. aeruginosa
antibiotic resistance
evolutionary biology
experimental evolution
infectious disease
integron
microbiology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
26 02 2021
26 02 2021
Historique:
received:
25
08
2020
accepted:
23
02
2021
pubmed:
27
2
2021
medline:
29
1
2022
entrez:
26
2
2021
Statut:
epublish
Résumé
Mobile integrons are widespread genetic platforms that allow bacteria to modulate the expression of antibiotic resistance cassettes by shuffling their position from a common promoter. Antibiotic stress induces the expression of an integrase that excises and integrates cassettes, and this unique recombination and expression system is thought to allow bacteria to 'evolve on demand' in response to antibiotic pressure. To test this hypothesis, we inserted a custom three-cassette integron into From urinary tract infections to bacterial pneumonia, many diseases can now be treated through a course of antibiotics. Yet bacteria have evolved to respond to this threat, gaining new antibiotic resistance genes that allow them to evade the drugs. Addressing this growing issue requires to either discover new antibiotics, or to stop resistance before it emerges – a strategy that can only work if scientists know exactly how this mechanism takes place. For bacteria, it is a waste of resources to produce the proteins that confer resistance if antibiotics are absent. In fact, doing so can decrease their chance to survive and reproduce. A genetic element known as an integron can help to manage that burden. This piece of genetic information is formed of a succession of ‘cassettes’ containing antibiotic resistance genes. More proteins are made from the genes present at the start of the integron, compared to the ones towards the end. When bacteria encounter antibiotics, an enzyme called integrase is activated, allowing the organisms to shuffle the order of their cassettes in the integron. It is thought – but not yet proven – that this mechanism helps bacteria to activate their resistance ‘on demand’. To find out, Souque et al. engineered the bacteria
Autres résumés
Type: plain-language-summary
(eng)
From urinary tract infections to bacterial pneumonia, many diseases can now be treated through a course of antibiotics. Yet bacteria have evolved to respond to this threat, gaining new antibiotic resistance genes that allow them to evade the drugs. Addressing this growing issue requires to either discover new antibiotics, or to stop resistance before it emerges – a strategy that can only work if scientists know exactly how this mechanism takes place. For bacteria, it is a waste of resources to produce the proteins that confer resistance if antibiotics are absent. In fact, doing so can decrease their chance to survive and reproduce. A genetic element known as an integron can help to manage that burden. This piece of genetic information is formed of a succession of ‘cassettes’ containing antibiotic resistance genes. More proteins are made from the genes present at the start of the integron, compared to the ones towards the end. When bacteria encounter antibiotics, an enzyme called integrase is activated, allowing the organisms to shuffle the order of their cassettes in the integron. It is thought – but not yet proven – that this mechanism helps bacteria to activate their resistance ‘on demand’. To find out, Souque et al. engineered the bacteria
Identifiants
pubmed: 33634790
doi: 10.7554/eLife.62474
pii: 62474
pmc: PMC8024014
doi:
pii:
Substances chimiques
Anti-Bacterial Agents
0
Banques de données
Dryad
['10.5061/dryad.rv15dv469']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/M011224/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 106918/Z/15/Z
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021, Souque et al.
Déclaration de conflit d'intérêts
CS, JE, RM No competing interests declared
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