Sleep-wake cycle disturbances and NeuN-altered expression in adult rats after cannabidiol treatments during adolescence.


Journal

Psychopharmacology
ISSN: 1432-2072
Titre abrégé: Psychopharmacology (Berl)
Pays: Germany
ID NLM: 7608025

Informations de publication

Date de publication:
Jun 2021
Historique:
received: 18 09 2020
accepted: 20 01 2021
pubmed: 27 2 2021
medline: 2 6 2021
entrez: 26 2 2021
Statut: ppublish

Résumé

The medical uses of cannabidiol (CBD), a constituent of the Cannabis sativa, have accelerated the legal and social acceptance for CBD-based medications but has also given the momentum for questioning whether the long-term use of CBD during the early years of life may induce adverse neurobiological effects in adulthood, including sleep disturbances. Given the critical window for neuroplasticity and neuro-functional changes that occur during stages of adolescence, we hypothesized that CBD might influence the sleep-wake cycle in adult rats after their exposure to CBD during the adolescence. Here, we investigated the effects upon behavior and neural activity in adulthood after long-term administrations of CBD in juvenile rats. We pre-treated juvenile rats with CBD (5 or 30 mg/Kg, daily) from post-natal day (PND) 30 and during 2 weeks. Following the treatments, the sleep-wake cycle and NeuN expression was analyzed at PND 80. We found that systemic injections of CBD (5 or 30 mg/Kg, i.p.) given to adolescent rats (post-natal day 30) for 14 days increased in adulthood the wakefulness and decreased rapid eye movement sleep during the lights-on period whereas across the lights-off period, wakefulness was diminished and slow wave sleep was enhanced. In addition, we found that adult animals that received CBD during the adolescence displayed disruptions in sleep rebound period after total sleep deprivation. Finally, we determined how the chronic administrations of CBD during the adolescence affected in the adulthood the NeuN expression in the suprachiasmatic nucleus, a sleep-related brain region. Our findings are relevant for interpreting results of adult rats that were chronically exposed to CBD during the adolescence and provide new insights into how CBD may impact the sleep-wake cycle and neuronal activity during developmental stages.

Identifiants

pubmed: 33635384
doi: 10.1007/s00213-021-05769-z
pii: 10.1007/s00213-021-05769-z
doi:

Substances chimiques

Cannabidiol 19GBJ60SN5

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1437-1447

Subventions

Organisme : University of California Institute for Mexico and the United States
ID : CN-17-19

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Auteurs

Eric Murillo-Rodríguez (E)

Laboratorio de Neurociencias Moleculares e Integrativas Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab Mérida, Km. 15.5 Carretera Mérida-Progreso, Int. Km. 2 Carretera a Chablekal, C.P. 97308, Mérida, Yucatán, Mexico. eric.murillo@anahuac.mx.
Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico. eric.murillo@anahuac.mx.

Diana Millán-Aldaco (D)

Depto. de Neurociencia Cognitiva, División de Neurociencias Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, Mexico.

Dorotea Cicconcelli (D)

Cognitive and Clinical Psychology Laboratory, Department of Human Sciences, European University of Rome, Rome, Italy.

Valentina Giorgetti (V)

Cognitive and Clinical Psychology Laboratory, Department of Human Sciences, European University of Rome, Rome, Italy.

Gloria Arankowsky-Sandoval (G)

Centro de Investigaciones Regionales "Dr. Hideyo Noguchi", Universidad Autónoma de Yucatán, Mérida, Yucatán, Mexico.

Jocelyne Alcaraz-Silva (J)

Laboratorio de Neurociencias Moleculares e Integrativas Escuela de Medicina, División Ciencias de la Salud, Universidad Anáhuac Mayab Mérida, Km. 15.5 Carretera Mérida-Progreso, Int. Km. 2 Carretera a Chablekal, C.P. 97308, Mérida, Yucatán, Mexico.
Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico.

Claudio Imperatori (C)

Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico.
Cognitive and Clinical Psychology Laboratory, Department of Human Sciences, European University of Rome, Rome, Italy.

Sérgio Machado (S)

Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico.
Laboratory of Physical Activity Neuroscience, Physical Activity Sciences Postgraduate Program, Salgado de Oliveira University, Niterói, Brazil.
Laboratory of Physical Activity Neuroscience, Neurodiversity Institute, Queimados, Rio de Janeiro, Brazil.

Henning Budde (H)

Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico.
Faculty of Human Sciences, Medical School Hamburg, Hamburg, Germany.

Pablo Torterolo (P)

Intercontinental Neuroscience Research Group, Mérida, Yucatán, Mexico.
Laboratorio de Neurobiología del Sueño, Departamento de Fisiología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.

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