The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis.
Arabidopsis
/ genetics
Arabidopsis Proteins
/ genetics
Co-Repressor Proteins
/ metabolism
DEAD-box RNA Helicases
/ metabolism
Gene Expression Regulation, Plant
Humans
Proto-Oncogene Proteins
/ metabolism
RNA Nucleotidyltransferases
/ genetics
RNA Stability
/ genetics
RNA, Messenger
/ metabolism
RNA, Small Interfering
/ metabolism
Ribonucleoproteins
/ metabolism
Saccharomyces cerevisiae
/ metabolism
Saccharomyces cerevisiae Proteins
/ metabolism
Nicotiana
/ genetics
Transcriptome
Uridine
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 02 2021
26 02 2021
Historique:
received:
12
05
2020
accepted:
19
01
2021
entrez:
27
2
2021
pubmed:
28
2
2021
medline:
16
3
2021
Statut:
epublish
Résumé
Uridylation is a widespread modification destabilizing eukaryotic mRNAs. Yet, molecular mechanisms underlying TUTase-mediated mRNA degradation remain mostly unresolved. Here, we report that the Arabidopsis TUTase URT1 participates in a molecular network connecting several translational repressors/decapping activators. URT1 directly interacts with DECAPPING 5 (DCP5), the Arabidopsis ortholog of human LSM14 and yeast Scd6, and this interaction connects URT1 to additional decay factors like DDX6/Dhh1-like RNA helicases. Nanopore direct RNA sequencing reveals a global role of URT1 in shaping poly(A) tail length, notably by preventing the accumulation of excessively deadenylated mRNAs. Based on in vitro and in planta data, we propose a model that explains how URT1 could reduce the accumulation of oligo(A)-tailed mRNAs both by favoring their degradation and because 3' terminal uridines intrinsically hinder deadenylation. Importantly, preventing the accumulation of excessively deadenylated mRNAs avoids the biogenesis of illegitimate siRNAs that silence endogenous mRNAs and perturb Arabidopsis growth and development.
Identifiants
pubmed: 33637717
doi: 10.1038/s41467-021-21382-2
pii: 10.1038/s41467-021-21382-2
pmc: PMC7910438
doi:
Substances chimiques
Arabidopsis Proteins
0
Co-Repressor Proteins
0
DCP5 protein, Arabidopsis
0
Proto-Oncogene Proteins
0
RNA, Messenger
0
RNA, Small Interfering
0
RNA-associated protein 55, human
0
Ribonucleoproteins
0
Saccharomyces cerevisiae Proteins
0
Scd6 protein, S cerevisiae
0
RNA Nucleotidyltransferases
EC 2.7.7.-
URT1 protein, Arabidopsis
EC 2.7.7.-
DDX6 protein, human
EC 3.6.1.-
DHH1 protein, S cerevisiae
EC 3.6.1.-
DEAD-box RNA Helicases
EC 3.6.4.13
Uridine
WHI7HQ7H85
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1298Références
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