The role of gut microbial community and metabolomic shifts in adaptive resistance of Atlantic killifish (Fundulus heteroclitus) to polycyclic aromatic hydrocarbons.
Adaptive resistance
Atlantic killifish
Gut metabolome
Gut microbes
Polycyclic aromatic hydrocarbons
Journal
The Science of the total environment
ISSN: 1879-1026
Titre abrégé: Sci Total Environ
Pays: Netherlands
ID NLM: 0330500
Informations de publication
Date de publication:
01 Jul 2021
01 Jul 2021
Historique:
received:
09
11
2020
revised:
12
02
2021
accepted:
13
02
2021
pubmed:
2
3
2021
medline:
30
4
2021
entrez:
1
3
2021
Statut:
ppublish
Résumé
Altered gut microbiomes may play a role in rapid evolution to anthropogenic change but remain poorly understood. Atlantic killifish (Fundulus heteroclitus) in the Elizabeth River, VA have evolved resistance to polycyclic aromatic hydrocarbons (PAHs) and provide a unique opportunity to examine the links between shifts in the commensal microbiome and organismal physiology associated with evolved resistance. Here, 16S rRNA sequence libraries derived from fish guts and sediments sampled from a highly PAH contaminated site revealed significant differences collected at similar samples from an uncontaminated site. Phylogenetic groups enriched in the libraries derived from PAH-resistant fish were dissimilar to their associated sediment libraries, suggesting the specific environment within the PAH-resistant fish intestine influence the gut microbiome composition. Gut metabolite analysis revealed shifts between PAH-resistant and non-resistant subpopulations. Notably, PAH-resistant fish exhibited reduced levels of tryptophan and increased levels of sphingolipids. Exposure to PAHs appears to impact several bacterial in the gut microbiome, particularly sphingolipid containing bacteria. Bacterial phylotypes known to include species containing sphingolipids were generally lower in the intestines of fish subpopulations exposed to high concentrations of PAHs, inferring a complex host-microbiome relationship. Overall, killifish microbial community shifts appear to be related to a suppression of overall metabolite level, indicating a potential role of the gut in organismal response to anthropogenic environmental change. These results on microbial and metabolomics shifts are potentially linked to altered bioenergetic phenotype observed in the same PAH-resistant killifish populations in other studies.
Identifiants
pubmed: 33647645
pii: S0048-9697(21)01022-6
doi: 10.1016/j.scitotenv.2021.145955
pmc: PMC8294123
mid: NIHMS1678364
pii:
doi:
Substances chimiques
Polycyclic Aromatic Hydrocarbons
0
RNA, Ribosomal, 16S
0
Water Pollutants, Chemical
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145955Subventions
Organisme : NIEHS NIH HHS
ID : P42 ES010356
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK097193
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Appl Environ Microbiol. 2013 Sep;79(17):5112-20
pubmed: 23793624
Arch Microbiol. 1997 Jan 29;167(1):11-8
pubmed: 9000336
Front Microbiol. 2016 Mar 14;7:285
pubmed: 27014209
Ecotoxicology. 2017 Apr;26(3):435-448
pubmed: 28213827
Front Biosci (Landmark Ed). 2011 Jan 01;16:1768-86
pubmed: 21196263
Curr Protoc Bioinformatics. 2011 Jun;Chapter 14:Unit 14.10
pubmed: 21633943
Cell. 2006 Feb 24;124(4):837-48
pubmed: 16497592
Science. 2016 Dec 09;354(6317):1305-1308
pubmed: 27940876
J Toxicol Environ Health B Crit Rev. 2015;18(6):259-98
pubmed: 26505693
Pharmacol Rev. 2013 Aug 01;65(4):1148-61
pubmed: 23908379
Environ Sci Pollut Res Int. 2014 Dec;21(24):13898-908
pubmed: 24374617
FEMS Microbiol Lett. 2006 Sep;262(2):148-57
pubmed: 16923069
Aquat Toxicol. 2014 Mar;148:16-26
pubmed: 24440964
Curr Opin Microbiol. 2018 Jun;43:92-99
pubmed: 29328957
Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2117-22
pubmed: 18252821
Int J Syst Evol Microbiol. 2013 Sep;63(Pt 9):3404-3408
pubmed: 23524358
Prog Lipid Res. 1990;29(1):1-43
pubmed: 2087478
Microb Ecol. 2009 Apr;57(3):455-68
pubmed: 18615233
FEMS Microbiol Ecol. 2014 Mar;87(3):704-14
pubmed: 24256454
Microbiologyopen. 2015 Oct;4(5):814-25
pubmed: 26242906
Med Sci Monit Basic Res. 2015 Apr 27;21:81-5
pubmed: 25913077
Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1311-1316
pubmed: 16738108
FEMS Microbiol Ecol. 2010 Sep;73(3):486-99
pubmed: 20550577
Microbiologyopen. 2013 Apr;2(2):259-75
pubmed: 23401293
Annu Rev Physiol. 2016;78:23-44
pubmed: 26474213
Mol Ecol. 2012 Jul;21(13):3363-78
pubmed: 22486918
Environ Microbiol. 2012 Sep;14(9):2511-25
pubmed: 22759205
Aquat Toxicol. 2010 Aug 1;99(1):33-41
pubmed: 20471113
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Nat Protoc. 2011 Jun;6(6):743-60
pubmed: 21637195
J Biol Chem. 2002 Jul 19;277(29):25851-4
pubmed: 12011102
J Gen Microbiol. 1986 Jun;132(6):1647-56
pubmed: 3543210
J Invertebr Pathol. 2016 Jan;133:12-9
pubmed: 26585302
Appl Environ Microbiol. 2013 Oct;79(19):5927-35
pubmed: 23872573
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1483-1487
pubmed: 15388699
J Biol Chem. 2011 Apr 15;286(15):12850-9
pubmed: 21321121
Front Microbiol. 2018 May 29;9:1124
pubmed: 29896183
PLoS One. 2014 Aug 21;9(8):e105592
pubmed: 25144201
Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2399-2405
pubmed: 17911317
J Inherit Metab Dis. 2010 Oct;33(5):469-77
pubmed: 20195903
Int J Syst Evol Microbiol. 2014 Dec;64(Pt 12):4160-4167
pubmed: 25256706
Water Res. 2018 Jan 1;128:102-110
pubmed: 29091801
Front Endocrinol (Lausanne). 2014 Apr 07;5:47
pubmed: 24778627
PLoS One. 2013 Jun 10;8(6):e65347
pubmed: 23762350
J Appl Microbiol. 2017 Mar;122(3):601-614
pubmed: 27992100
FEMS Immunol Med Microbiol. 2008 Mar;52(2):145-54
pubmed: 18081845
Anaerobe. 1995 Apr;1(2):135-9
pubmed: 16887518
Arch Environ Contam Toxicol. 2017 Jul;73(1):12-18
pubmed: 28695262
Aquat Toxicol. 2017 Sep;190:162-173
pubmed: 28728047
Front Immunol. 2017 May 15;8:559
pubmed: 28555138
Ecotoxicology. 2019 Nov;28(9):1126-1135
pubmed: 31620948
Toxicol Sci. 2002 Jul;68(1):69-81
pubmed: 12075112
Sci Rep. 2016 Dec 14;6:39054
pubmed: 27966609
Toxicol Pathol. 2016 Jul;44(5):738-48
pubmed: 26992886
Environ Sci Technol. 2013 Sep 17;47(18):10556-66
pubmed: 24003986
Nat Med. 2016 Jun;22(6):586-97
pubmed: 27158906
J Lipid Res. 2009 Apr;50 Suppl:S91-6
pubmed: 19017611
Aquat Toxicol. 2018 Jul;200:73-82
pubmed: 29727773
Nucleic Acids Res. 2012 Jul;40(Web Server issue):W127-33
pubmed: 22553367
Nat Commun. 2014 Nov 20;5:5492
pubmed: 25411059
J Dairy Sci. 2018 Jan;101(1):812-819
pubmed: 29103713
Chem Phys Lipids. 2000 Jun;106(1):1-29
pubmed: 10878232
Behav Brain Res. 2016 Sep 15;311:219-227
pubmed: 27217102
PLoS One. 2016 Jan 28;11(1):e0148135
pubmed: 26820139
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
J Gen Appl Microbiol. 2009 Jun;55(3):201-5
pubmed: 19590147
J Lipid Res. 2014 Dec;55(12):2620-33
pubmed: 25332432
Microb Ecol. 2016 Feb;71(2):387-400
pubmed: 26337826
Nucleic Acids Res. 2015 Jul 1;43(W1):W251-7
pubmed: 25897128
Environ Sci Technol. 2017 Aug 1;51(15):8763-8772
pubmed: 28682633
Ecotoxicology. 2011 Nov;20(8):1890-9
pubmed: 21706406