Quantitative analysis of dense-core vesicle fusion in rodent CNS neurons.

Algorithms Animals Dense Core Vesicles / genetics Genes, Reporter Hippocampus / metabolism Mice Neurons / metabolism Synapses / genetics
Cell culture Cell membrane Microscopy Molecular/chemical probes Neuroscience

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
19 03 2021
Historique:
entrez: 4 3 2021
pubmed: 5 3 2021
medline: 5 3 2021
Statut: epublish

Résumé

Neuropeptides are essential signaling molecules secreted by dense-core vesicles (DCVs). They contribute to information processing in the brain, controlling a variety of physiological conditions. Defective neuropeptide signaling is implicated in several psychiatric disorders. Here, we provide a protocol for the quantitative analysis of DCV fusion events in rodent neurons using pH-sensitive DCV fusion probes and custom-written analysis algorithms. This method can be used to study DCV fusion mechanisms and is easily adapted to investigate fusion principles of other secretory organelles. For complete details on the use and execution of this protocol, please refer to Persoon et al. (2019).

Identifiants

DOI: 10.1016/j.xpro.2021.100325 PMID: 33659902 PMC: PMC7890040
pubmed: 33659902
doi: 10.1016/j.xpro.2021.100325
pii: S2666-1667(21)00032-0
pmc: PMC7890040
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100325

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

J Neurosci. 2008 Dec 10;28(50):13435-47
pubmed: 19074017
J Neurosci. 2009 Jan 7;29(1):23-37
pubmed: 19129381
Nat Protoc. 2012 Jun 21;7(7):1351-65
pubmed: 22722370
J Neurosci. 2004 Jul 21;24(29):6629-37
pubmed: 15269275
Neuron. 2019 Dec 18;104(6):1065-1080.e12
pubmed: 31679900
J Cell Biol. 2012 Dec 10;199(6):883-91
pubmed: 23229896
EMBO J. 2018 Oct 15;37(20):
pubmed: 30185408
Cell. 2011 Jan 21;144(2):282-95
pubmed: 21241895
Science. 1996 Apr 12;272(5259):263-7
pubmed: 8602510
EMBO J. 2001 Nov 1;20(21):5887-97
pubmed: 11689429
Nat Biotechnol. 2002 Jan;20(1):87-90
pubmed: 11753368
Cell Mol Life Sci. 2014 Aug;71(15):2787-814
pubmed: 24442513

Auteurs

Alessandro Moro (A)

Department of Clinical Genetics, UMC Amsterdam, the Netherlands.

Rein I Hoogstraaten (RI)

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
Department of Clinical Genetics, UMC Amsterdam, the Netherlands.

Claudia M Persoon (CM)

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
Department of Clinical Genetics, UMC Amsterdam, the Netherlands.

Matthijs Verhage (M)

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.
Department of Clinical Genetics, UMC Amsterdam, the Netherlands.

Ruud F Toonen (RF)

Department of Functional Genomics, Center for Neurogenomics and Cognitive Research (CNCR), Vrije Universiteit (VU) Amsterdam, de Boelelaan 1085, 1081 HV Amsterdam, the Netherlands.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

Selecting optimal software code descriptors-The case of Java.

Yegor Bugayenko, Zamira Kholmatova, Artem Kruglov et al.
1.00
Software Algorithms Programming Languages

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Algorithmes › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Eucaryotes › Animaux › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Vésicules de sécrétion › Vésicules à coeur dense › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Gènes rapporteurs › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Prosencéphale › Télencéphale › Cerveau › Cortex cérébral › Hippocampe › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Cellules › Neurones › Quantitative analysis of dense-core vesicle...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Synapses › Quantitative analysis of dense-core vesicle...