CD74 is a regulator of hematopoietic stem cell maintenance.
Adult
Animals
Antigens, Differentiation, B-Lymphocyte
/ genetics
Bone Marrow Cells
/ metabolism
Bone Marrow Transplantation
/ methods
Cell Lineage
Female
Healthy Volunteers
Hematopoietic Stem Cells
/ cytology
Histocompatibility Antigens Class II
/ genetics
Humans
Intramolecular Oxidoreductases
/ metabolism
Macrophage Migration-Inhibitory Factors
/ metabolism
Male
Mice
Mice, Inbred C57BL
Signal Transduction
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
08
04
2020
accepted:
29
01
2021
revised:
16
03
2021
pubmed:
5
3
2021
medline:
27
7
2021
entrez:
4
3
2021
Statut:
epublish
Résumé
Hematopoietic stem and progenitor cells (HSPCs) are a small population of undifferentiated cells that have the capacity for self-renewal and differentiate into all blood cell lineages. These cells are the most useful cells for clinical transplantations and for regenerative medicine. So far, it has not been possible to expand adult hematopoietic stem cells (HSCs) without losing their self-renewal properties. CD74 is a cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF), and its mRNA is known to be expressed in HSCs. Here, we demonstrate that mice lacking CD74 exhibit an accumulation of HSCs in the bone marrow (BM) due to their increased potential to repopulate and compete for BM niches. Our results suggest that CD74 regulates the maintenance of the HSCs and CD18 expression. Its absence leads to induced survival of these cells and accumulation of quiescent and proliferating cells. Furthermore, in in vitro experiments, blocking of CD74 elevated the numbers of HSPCs. Thus, we suggest that blocking CD74 could lead to improved clinical insight into BM transplant protocols, enabling improved engraftment.
Identifiants
pubmed: 33661886
doi: 10.1371/journal.pbio.3001121
pii: PBIOLOGY-D-20-00918
pmc: PMC7963458
doi:
Substances chimiques
Antigens, Differentiation, B-Lymphocyte
0
Histocompatibility Antigens Class II
0
Macrophage Migration-Inhibitory Factors
0
invariant chain
0
Intramolecular Oxidoreductases
EC 5.3.-
MIF protein, human
EC 5.3.2.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3001121Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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