The effect of gut microbiota on the intestinal lipidome of mice.

Animals Colon Fatty Acids Gastrointestinal Microbiome Gastrointestinal Tract Lipidomics Mice
Cholesterol Colon Germfree Gut microbiota Ileum Lipidomics PUFA Phosphatidylcholine

Journal

International journal of medical microbiology : IJMM
ISSN: 1618-0607
Titre abrégé: Int J Med Microbiol
Pays: Germany
ID NLM: 100898849

Informations de publication

Date de publication:
Apr 2021
Historique:
received: 15 09 2020
revised: 01 02 2021
accepted: 23 02 2021
pubmed: 5 3 2021
medline: 15 5 2021
entrez: 4 3 2021
Statut: ppublish

Résumé

Gut microbiota significantly influence the plasma and liver lipidome. An interconnecting metabolite is acetate generated after degradation and fermentation of dietary fiber by the gut microbiota, which is metabolized in the liver into longer chain fatty acids and complex lipids reaching the circulation. Whether these systemic changes are accompanied by alternations of the intestinal lipidome is unclear. Therefore, we quantified glycerophospholipids, sphingolipids and sterols in ileum and colon, the two segments containing the highest densities of microbes in the gastrointestinal tract, of germfree and specific pathogen free mice using mass spectrometry-based lipidomics. We found that the presence of gut microbes lowers the free cholesterol content in colon while elevating phosphatidylcholine levels. Further, PUFA-containing phosphatidylcholine and -ethanolamine fractions are increased in ileum and colon of germfree compared to SPF mice. A total fatty acid analysis by GC-MS revealed higher levels of arachidonic and docosahexaenoic acid in the ileum of germfree mice indicating that the gut microbiota inhibits PUFA metabolism in the small intestine.

Identifiants

DOI: 10.1016/j.ijmm.2021.151488 PMID: 33662870
pubmed: 33662870
pii: S1438-4221(21)00017-5
doi: 10.1016/j.ijmm.2021.151488
pii:
doi:

Substances chimiques

Fatty Acids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

151488

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier GmbH.. All rights reserved.

Auteurs

Gerhard Liebisch (G)

Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany. Electronic address: gerhard.liebisch@ukr.de.

Johannes Plagge (J)

ZIEL - Institute for Food & Health, Research Group Lipid Metabolism, Technical University of Munich, Freising, Germany.

Marcus Höring (M)

Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany.

Claudine Seeliger (C)

ZIEL - Institute for Food & Health, Research Group Lipid Metabolism, Technical University of Munich, Freising, Germany.

Josef Ecker (J)

ZIEL - Institute for Food & Health, Research Group Lipid Metabolism, Technical University of Munich, Freising, Germany. Electronic address: josef.ecker@tum.de.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The effect of gut microbiota on the intestinal...
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif inférieur › Gros intestin › Côlon › The effect of gut microbiota on the intestinal...
questionsmedicales.fr › Lipides › Acides gras › The effect of gut microbiota on the intestinal...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › The effect of gut microbiota on the intestinal...
questionsmedicales.fr › Système digestif › Tube digestif › The effect of gut microbiota on the intestinal...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Métabolomique › Lipidomique › The effect of gut microbiota on the intestinal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › The effect of gut microbiota on the intestinal...