Establishment and evaluation of an indirect ELISA for detection of antibodies to goat Klebsiella pneumonia.
Clinical veterinary application
Goat Klebsiella pneumonia
Indirect ELISA detection method
Optimal working conditions
Polyclonal antibodies
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
05 Mar 2021
05 Mar 2021
Historique:
received:
06
08
2020
accepted:
25
02
2021
entrez:
5
3
2021
pubmed:
6
3
2021
medline:
10
8
2021
Statut:
epublish
Résumé
Klebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are little systematic methods for detection of goat Klebsiella pneumoniae in livestock production. In this study, we developed a Klebsiella pneumoniae goat polyclonal antibody and established an indirect ELISA method to detect the Klebsiella pneumoniae. After screening and optimizing the conditions for detection, we determined the optimal working dilutions of the coated-bacterial antigen, the polyclonal antibody, and the enzyme-labeled secondary antibody that were 1:800 (2.99 × 10 Taken together, we successfully established an indirect ELISA method for detecting antibodies against Klebsiella pneumoniae in goats, which can be applied in production.
Sections du résumé
BACKGROUND
BACKGROUND
Klebsiella pneumonia, a Gram-negative bacterium belonging to the genus Enterobacter, causes many human and livestock diseases. Notably, infected goats may develop pneumonia, septicemia, which can lead to occasional death, resulting in great economic losses in goat-farming industry. However, there are little systematic methods for detection of goat Klebsiella pneumoniae in livestock production.
RESULTS
RESULTS
In this study, we developed a Klebsiella pneumoniae goat polyclonal antibody and established an indirect ELISA method to detect the Klebsiella pneumoniae. After screening and optimizing the conditions for detection, we determined the optimal working dilutions of the coated-bacterial antigen, the polyclonal antibody, and the enzyme-labeled secondary antibody that were 1:800 (2.99 × 10
CONCLUSIONS
CONCLUSIONS
Taken together, we successfully established an indirect ELISA method for detecting antibodies against Klebsiella pneumoniae in goats, which can be applied in production.
Identifiants
pubmed: 33663505
doi: 10.1186/s12917-021-02820-1
pii: 10.1186/s12917-021-02820-1
pmc: PMC7934495
doi:
Substances chimiques
Antibodies, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107Subventions
Organisme : the National Key Research and Development Program of China
ID : 2016YFD0500905
Organisme : the Modern Agricultural Industry Technology System Foundation of Shandong Province
ID : SDAIT-10-06
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