Mutanofactin promotes adhesion and biofilm formation of cariogenic Streptococcus mutans.
Bacterial Adhesion
/ drug effects
Biofilms
/ drug effects
Biological Factors
/ biosynthesis
Computational Biology
/ methods
DNA
/ genetics
Dental Caries
/ microbiology
Gene Expression Regulation, Bacterial
Genes, Bacterial
Humans
Hydrophobic and Hydrophilic Interactions
Multigene Family
Peptide Biosynthesis, Nucleic Acid-Independent
Protein Binding
Secondary Metabolism
/ genetics
Streptococcus mutans
/ genetics
Journal
Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
05
06
2020
accepted:
21
01
2021
pubmed:
6
3
2021
medline:
24
6
2021
entrez:
5
3
2021
Statut:
ppublish
Résumé
Cariogenic Streptococcus mutans is known as a predominant etiological agent of dental caries due to its exceptional capacity to form biofilms. From strains of S. mutans isolated from dental plaque, we discovered, in the present study, a polyketide/nonribosomal peptide biosynthetic gene cluster, muf, which directly correlates with a strong biofilm-forming capability. We then identified the muf-associated bioactive product, mutanofactin-697, which contains a new molecular scaffold, along with its biosynthetic logic. Further mode-of-action studies revealed that mutanofactin-697 binds to S. mutans cells and also extracellular DNA, increases bacterial hydrophobicity, and promotes bacterial adhesion and subsequent biofilm formation. Our findings provided an example of a microbial secondary metabolite promoting biofilm formation via a physicochemical approach, highlighting the importance of secondary metabolism in mediating critical processes related to the development of dental caries.
Identifiants
pubmed: 33664521
doi: 10.1038/s41589-021-00745-2
pii: 10.1038/s41589-021-00745-2
doi:
Substances chimiques
Biological Factors
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
576-584Commentaires et corrections
Type : CommentIn
Type : CommentIn
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