Revisiting a 'simple' fungal metabolic pathway reveals redundancy, complexity and diversity.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
17
02
2021
received:
06
12
2020
accepted:
17
02
2021
pubmed:
6
3
2021
medline:
28
1
2022
entrez:
5
3
2021
Statut:
ppublish
Résumé
Next to d-glucose, the pentoses l-arabinose and d-xylose are the main monosaccharide components of plant cell wall polysaccharides and are therefore of major importance in biotechnological applications that use plant biomass as a substrate. Pentose catabolism is one of the best-studied pathways of primary metabolism of Aspergillus niger, and an initial outline of this pathway with individual enzymes covering each step of the pathway has been previously established. However, although growth on l-arabinose and/or d-xylose of most pentose catabolic pathway (PCP) single deletion mutants of A. niger has been shown to be negatively affected, it was not abolished, suggesting the involvement of additional enzymes. Detailed analysis of the single deletion mutants of the known A. niger PCP genes led to the identification of additional genes involved in the pathway. These results reveal a high level of complexity and redundancy in this pathway, emphasizing the need for a comprehensive understanding of metabolic pathways before entering metabolic engineering of such pathways for the generation of more efficient fungal cell factories.
Identifiants
pubmed: 33666344
doi: 10.1111/1751-7915.13790
pmc: PMC8601170
doi:
Substances chimiques
Pentoses
0
Xylose
A1TA934AKO
Arabinose
B40ROO395Z
Banques de données
GENBANK
['SRP225864', 'SRP225877', 'SRP242739', 'SRP242757', 'SRP242763', 'SRP242774', 'SRP242787', 'SRP242846', 'SRP242859', 'SRP242894', 'SRP242906', 'SRP242928', 'SRP242942', 'SRP242953']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2525-2537Informations de copyright
© 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
Références
Biotechnol Lett. 2013 May;35(5):769-77
pubmed: 23436125
Appl Environ Microbiol. 2011 Sep;77(17):5988-94
pubmed: 21742908
Genome Biol. 2014;15(12):550
pubmed: 25516281
Stud Mycol. 2011 Jun 30;69(1):31-8
pubmed: 21892241
Eukaryot Cell. 2013 Mar;12(3):390-8
pubmed: 23291620
N Biotechnol. 2016 Dec 25;33(6):834-841
pubmed: 27469436
Stud Mycol. 2018 Sep;91:61-78
pubmed: 30425417
BMC Microbiol. 2009 Aug 12;9:166
pubmed: 19674460
Appl Environ Microbiol. 2004 Jul;70(7):3954-9
pubmed: 15240269
Eur J Biochem. 2001 Oct;268(20):5414-23
pubmed: 11606204
Mol Microbiol. 1998 Jan;27(1):131-42
pubmed: 9466262
Eukaryot Cell. 2003 Oct;2(5):867-75
pubmed: 14555469
Genome Biol. 2017 Feb 14;18(1):28
pubmed: 28196534
Nat Genet. 2018 Dec;50(12):1688-1695
pubmed: 30349117
Microbiol Res. 2020 Feb 7;234:126426
pubmed: 32062364
Sci Rep. 2017 Sep 27;7(1):12356
pubmed: 28955038
J Biol Chem. 2010 Jul 30;285(31):23622-8
pubmed: 20511228
Microbiol Mol Biol Rev. 2001 Dec;65(4):497-522, table of contents
pubmed: 11729262
Appl Microbiol Biotechnol. 2011 Mar;89(6):1665-73
pubmed: 21212945
Mol Microbiol. 2000 Apr;36(1):193-200
pubmed: 10760176
PLoS One. 2015 Jul 15;10(7):e0133085
pubmed: 26177455
Bioinformatics. 2012 Jun 15;28(12):1647-9
pubmed: 22543367
FEMS Microbiol Lett. 1994 Oct 15;123(1-2):83-90
pubmed: 7988903
Mol Microbiol. 2003 Jul;49(1):131-41
pubmed: 12823816
FEBS Lett. 2013 May 2;587(9):1346-52
pubmed: 23499935
FEBS Lett. 2012 Feb 17;586(4):378-83
pubmed: 22245674
Nucleic Acids Res. 2014 Jan;42(Database issue):D699-704
pubmed: 24297253
Mol Biol Evol. 2013 Dec;30(12):2725-9
pubmed: 24132122
Res Microbiol. 1999 May;150(4):281-5
pubmed: 10376490
BMC Genomics. 2017 Nov 23;18(1):900
pubmed: 29169319
FEBS Lett. 2010 Aug 20;584(16):3540-4
pubmed: 20654618
PLoS One. 2018 Aug 24;13(8):e0202868
pubmed: 30142205
Biotechnol J. 2013 Aug;8(8):905-11
pubmed: 23713061