FOXC1 Downregulates Nanog Expression by Recruiting HDAC2 to Its Promoter in F9 Cells Treated by Retinoic Acid.
Animals
Antineoplastic Agents
/ pharmacology
Embryonal Carcinoma Stem Cells
/ drug effects
Epigenesis, Genetic
Forkhead Transcription Factors
/ genetics
Gene Expression Regulation, Neoplastic
/ drug effects
HEK293 Cells
Histone Deacetylase 2
/ genetics
Humans
Mice
NIH 3T3 Cells
Nanog Homeobox Protein
/ genetics
Promoter Regions, Genetic
Tretinoin
/ pharmacology
ARTA
F9 cells
FOXC1 interactome
HDAC2
Nanog
promoter analysis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
24 Feb 2021
24 Feb 2021
Historique:
received:
23
12
2020
revised:
16
02
2021
accepted:
20
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
30
4
2021
Statut:
epublish
Résumé
FOXC1, a transcription factor involved in cell differentiation and embryogenesis, is demonstrated to be a negative regulator of Nanog in this study. FOXC1 is up-regulated in retinoic acid-induced differentiation of F9 Embryonal Carcinoma (EC) cells; furthermore, FOXC1 specifically inhibits the core pluripotency factor Nanog by binding to the proximal promoter. Overexpression of FOXC1 in F9 or knockdown in 3T3 results in the down-regulation or up-regulation of Nanog mRNA and proteins, respectively. In order to explain the mechanism by which FOXC1 inhibits Nanog expression, we identified the co-repressor HDAC2 from the FOXC1 interactome. FOXC1 recruits HDAC2 to Nanog promoter to decrease H3K27ac enrichment, resulting in transcription inhibition of Nanog. To the best of our knowledge, this is the first report that FOXC1 is involved in the epigenetic regulation of gene expression.
Identifiants
pubmed: 33668324
pii: ijms22052255
doi: 10.3390/ijms22052255
pmc: PMC7956269
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
FOXC1 protein, human
0
Forkhead Transcription Factors
0
NANOG protein, human
0
Nanog Homeobox Protein
0
Tretinoin
5688UTC01R
HDAC2 protein, human
EC 3.5.1.98
Histone Deacetylase 2
EC 3.5.1.98
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31872355
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