Protective Role of Sphingomyelin in Eye Lens Cell Membrane Model against Oxidative Stress.
aging
atomic force microscopy
cold physical plasma
electrochemistry
eye lens cell membrane
mass spectrometry
oxidized lipids
sphingomyelin
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
13 02 2021
13 02 2021
Historique:
received:
27
01
2021
revised:
09
02
2021
accepted:
11
02
2021
entrez:
6
3
2021
pubmed:
7
3
2021
medline:
20
7
2021
Statut:
epublish
Résumé
In the eye lens cell membrane, the lipid composition changes during the aging process: the proportion of sphingomyelins (SM) increases, that of phosphatidylcholines decreases. To investigate the protective role of the SMs in the lens cell membrane against oxidative damage, analytical techniques such as electrochemistry, high-resolution mass spectrometry (HR-MS), and atomic force microscopy (AFM) were applied. Supported lipid bilayers (SLB) were prepared to mimic the lens cell membrane with different fractions of PLPC/SM (PLPC: 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine). The SLBs were treated with cold physical plasma. A protective effect of 30% and 44% in the presence of 25%, and 75% SM in the bilayer was observed, respectively. PLPC and SM oxidation products were determined via HR-MS for SLBs after plasma treatment. The yield of fragments gradually decreased as the SM ratio increased. Topographic images obtained by AFM of PLPC-bilayers showed SLB degradation and pore formation after plasma treatment, no degradation was observed in PLPC/SM bilayers. The results of all techniques confirm the protective role of SM in the membrane against oxidative damage and support the idea that the SM content in lens cell membrane is increased during aging in the absence of effective antioxidant systems to protect the eye from oxidative damage and to prolong lens transparency.
Identifiants
pubmed: 33668553
pii: biom11020276
doi: 10.3390/biom11020276
pmc: PMC7918908
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Plasma Gases
0
Sphingomyelins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 03Z22DN12
Organisme : Deutsche Forschungsgemeinschaft
ID : 231396381/GRK1947
Organisme : Bundesministerium für Bildung und Forschung
ID : 03Z22CI1
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