Ultra high frequency ultrasonography to distinguish ganglionic from aganglionic bowel wall in Hirschsprung disease: A first report.


Journal

Journal of pediatric surgery
ISSN: 1531-5037
Titre abrégé: J Pediatr Surg
Pays: United States
ID NLM: 0052631

Informations de publication

Date de publication:
Dec 2021
Historique:
received: 09 11 2020
revised: 21 01 2021
accepted: 04 02 2021
pubmed: 8 3 2021
medline: 30 11 2021
entrez: 7 3 2021
Statut: ppublish

Résumé

In Hirschsprung disease (HD) surgery, confirming ganglionic bowel is essential. A faster diagnostic method than the current frozen biopsy is desirable. This study investigated whether aganglionic and ganglionic intestinal wall can be distinguished from each other by ultra high frequency ultrasound (UHF ultrasound). In an HD center during 2019, intestinal walls of recto-sigmoid specimens from HD patients were examined ex vivo with a 70 MHz UHF ultrasound transducer. Data from four sites were described. Histopathologic analysis was compared to the ultrasonography outcome at each site. Each patient's specimen served as its own control. 11 resected recto-sigmoid specimens (median 20 cm long [range 6.5-33]) with transition zones of 5 cm (2-11 cm) were taken from children aged 22 days (13-48) weighing 3668 g (3500-5508); 44 key sites were analyzed. There was full concordance for 42/44 (95%) key sites and 10 of 11 (91%) specimens. The specimen with discordance of two key sites contained a segment of aganglionosis (3 cm) and a transition zone (1 cm): the site discordance was limited to the transition zone ends. This first report on UHF ultrasound in recto-sigmoid HD shows promising results in identifying aganglionosis, transition zones and ganglionic bowel. Further in vivo studies are required.

Sections du résumé

BACKGROUND/PURPOSE OBJECTIVE
In Hirschsprung disease (HD) surgery, confirming ganglionic bowel is essential. A faster diagnostic method than the current frozen biopsy is desirable. This study investigated whether aganglionic and ganglionic intestinal wall can be distinguished from each other by ultra high frequency ultrasound (UHF ultrasound).
METHODS METHODS
In an HD center during 2019, intestinal walls of recto-sigmoid specimens from HD patients were examined ex vivo with a 70 MHz UHF ultrasound transducer. Data from four sites were described. Histopathologic analysis was compared to the ultrasonography outcome at each site. Each patient's specimen served as its own control.
RESULTS RESULTS
11 resected recto-sigmoid specimens (median 20 cm long [range 6.5-33]) with transition zones of 5 cm (2-11 cm) were taken from children aged 22 days (13-48) weighing 3668 g (3500-5508); 44 key sites were analyzed. There was full concordance for 42/44 (95%) key sites and 10 of 11 (91%) specimens. The specimen with discordance of two key sites contained a segment of aganglionosis (3 cm) and a transition zone (1 cm): the site discordance was limited to the transition zone ends.
CONCLUSIONS CONCLUSIONS
This first report on UHF ultrasound in recto-sigmoid HD shows promising results in identifying aganglionosis, transition zones and ganglionic bowel. Further in vivo studies are required.

Identifiants

pubmed: 33676743
pii: S0022-3468(21)00119-6
doi: 10.1016/j.jpedsurg.2021.02.011
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2281-2285

Informations de copyright

Copyright © 2021. Published by Elsevier Inc.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare no conflict of interests.

Auteurs

Christina Granéli (C)

Department of Pediatric Surgery, Skåne University Hospital Lund, Lund University, Lund, Sweden.

Tobias Erlöv (T)

Department of Biomedical Engineering, The Faculty of Engineering, Lund University, Lund, Sweden.

Rodrigo Munoz Mitev (RM)

Department of Clinical Genetics and Oncology-Pathology, Skåne University Hospital Lund, Lund University, Lund, Sweden.

Ioanna Kasselaki (I)

Department of Clinical Genetics and Oncology-Pathology, Skåne University Hospital Lund, Lund University, Lund, Sweden.

Kristine Hagelsteen (K)

Department of Pediatric Surgery, Skåne University Hospital Lund, Lund University, Lund, Sweden.

David Gisselsson (D)

Department of Clinical Genetics and Oncology-Pathology, Skåne University Hospital Lund, Lund University, Lund, Sweden.

Tomas Jansson (T)

Department of Clinical Sciences Lund/Biomedical Engineering, Lund University, Lund, Sweden; Clinical Engineering Skåne, Digitalisering IT/MT, Region Skåne, Sweden.

Magnus Cinthio (M)

Department of Biomedical Engineering, The Faculty of Engineering, Lund University, Lund, Sweden.

Pernilla Stenström (P)

Department of Pediatric Surgery, Skåne University Hospital Lund, Lund University, Lund, Sweden. Electronic address: pernilla.stenstrom@med.lu.se.

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