Pneumocystis jirovecii among patients with cystic fibrosis and their household members.
Adolescent
Adult
Child
Cystic Fibrosis
/ complications
Family Characteristics
Female
Genotype
Humans
Infectious Disease Transmission, Vertical
Male
Pilot Projects
Pneumococcal Infections
/ transmission
Pneumocystis carinii
/ genetics
Pneumonia, Pneumocystis
/ transmission
Polymerase Chain Reaction
/ methods
Pseudomonas Infections
/ transmission
Pseudomonas aeruginosa
/ genetics
Streptococcus pneumoniae
/ genetics
Young Adult
Pneumocystis jirovecii
cystic fibrosis
disease transmission
polymerase chain reaction
Journal
Medical mycology
ISSN: 1460-2709
Titre abrégé: Med Mycol
Pays: England
ID NLM: 9815835
Informations de publication
Date de publication:
03 Sep 2021
03 Sep 2021
Historique:
received:
23
10
2020
revised:
06
02
2021
accepted:
09
02
2021
pubmed:
12
3
2021
medline:
23
11
2021
entrez:
11
3
2021
Statut:
ppublish
Résumé
We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection. This study suggests for the first time the possible transmission of Pneumocystis in the family environment of patients with cystic fibrosis, indicating that patients and their household members are reservoirs and possible sources of this infection.
Identifiants
pubmed: 33693837
pii: 6166790
doi: 10.1093/mmy/myab010
pmc: PMC8754488
doi:
Types de publication
Comparative Study
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
849-854Subventions
Organisme : CYTED
ID : 212RT0450
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.
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