BamA forms a translocation channel for polypeptide export across the bacterial outer membrane.
BAM
BamA
Omp85 superfamily
autotransporter
outer membrane protein
protein folding
secretion
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
06 05 2021
06 05 2021
Historique:
received:
19
10
2020
revised:
05
01
2021
accepted:
18
02
2021
pubmed:
12
3
2021
medline:
16
6
2021
entrez:
11
3
2021
Statut:
ppublish
Résumé
The β-barrel assembly machine (BAM) integrates β-barrel proteins into the outer membrane (OM) of Gram-negative bacteria. An essential BAM subunit (BamA) catalyzes integration by promoting the formation of a hybrid-barrel intermediate state between its own β-barrel domain and that of its client proteins. Here we show that in addition to catalyzing the integration of β-barrel proteins, BamA functions as a polypeptide export channel. In vivo structural mapping via intermolecular disulfide crosslinking showed that the extracellular "passenger" domain of a member of the "autotransporter" superfamily of virulence factors traverses the OM through the BamA β-barrel lumen. Furthermore, we demonstrate that a highly conserved residue within autotransporter β-barrels is required to position the passenger inside BamA to initiate translocation and that during translocation, the passenger stabilizes the hybrid-barrel state. Our results not only establish a new function for BamA but also unify the divergent functions of BamA and other "Omp85" superfamily transporters.
Identifiants
pubmed: 33705710
pii: S1097-2765(21)00133-7
doi: 10.1016/j.molcel.2021.02.023
pmc: PMC8106670
mid: NIHMS1683470
pii:
doi:
Substances chimiques
Bacterial Outer Membrane Proteins
0
BamA protein, E coli
0
Escherichia coli Proteins
0
Tryptophan
8DUH1N11BX
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2000-2012.e3Subventions
Organisme : Intramural NIH HHS
ID : ZIA DK052037
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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