APTES monolayer coverage on self-assembled magnetic nanospheres for controlled release of anticancer drug Nintedanib.
Antineoplastic Agents
/ chemistry
Cell Death
/ drug effects
Cell Line, Tumor
Cell Survival
/ drug effects
Delayed-Action Preparations
/ pharmacology
Drug Liberation
Humans
Indoles
/ chemistry
Magnetite Nanoparticles
/ chemistry
Nanospheres
/ chemistry
Photoelectron Spectroscopy
Propylamines
/ chemistry
Protons
Silanes
/ chemistry
Spectroscopy, Fourier Transform Infrared
Temperature
X-Ray Diffraction
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 03 2021
11 03 2021
Historique:
received:
10
10
2020
accepted:
16
02
2021
entrez:
12
3
2021
pubmed:
13
3
2021
medline:
15
12
2021
Statut:
epublish
Résumé
The use of an appropriate delivery system capable of protecting, translocating, and selectively releasing therapeutic moieties to desired sites can promote the efficacy of an active compound. In this work, we have developed a nanoformulation which preserves its magnetization to load a model anticancerous drug and to explore the controlled release of the drug in a cancerous environment. For the preparation of the nanoformulation, self-assembled magnetic nanospheres (MNS) made of superparamagnetic iron oxide nanoparticles were grafted with a monolayer of (3-aminopropyl)triethoxysilane (APTES). A direct functionalization strategy was used to avoid the loss of the MNS magnetization. The successful preparation of the nanoformulation was validated by structural, microstructural, and magnetic investigations. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR) were used to establish the presence of APTES on the MNS surface. The amine content quantified by a ninhydrin assay revealed the monolayer coverage of APTES over MNS. The monolayer coverage of APTES reduced only negligibly the saturation magnetization from 77 emu/g (for MNS) to 74 emu/g (for MNS-APTES). Detailed investigations of the thermoremanent magnetization were carried out to assess the superparamagnetism in the MNS. To make the nanoformulation pH-responsive, the anticancerous drug Nintedanib (NTD) was conjugated with MNS-APTES through the acid liable imine bond. At pH 5.5, which mimics a cancerous environment, a controlled release of 85% in 48 h was observed. On the other hand, prolonged release of NTD was found at physiological conditions (i.e., pH 7.4). In vitro cytotoxicity study showed dose-dependent activity of MNS-APTES-NTD for human lung cancer cells L-132. About 75% reduction in cellular viability for a 100 μg/mL concentration of nanoformulation was observed. The nanoformulation designed using MNS and monolayer coverage of APTES has potential in cancer therapy as well as in other nanobiological applications.
Identifiants
pubmed: 33707549
doi: 10.1038/s41598-021-84770-0
pii: 10.1038/s41598-021-84770-0
pmc: PMC7952395
doi:
Substances chimiques
Antineoplastic Agents
0
Delayed-Action Preparations
0
Indoles
0
Magnetite Nanoparticles
0
Propylamines
0
Protons
0
Silanes
0
nintedanib
G6HRD2P839
amino-propyl-triethoxysilane
L8S6UBW552
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5674Références
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