The use of fluorescence correlation spectroscopy to monitor cell surface β2-adrenoceptors at low expression levels in human embryonic stem cell-derived cardiomyocytes and fibroblasts.
Adrenergic beta-2 Receptor Agonists
/ pharmacology
Cell Differentiation
Embryonic Stem Cells
/ physiology
Fibroblasts
/ physiology
Fluorescent Dyes
/ chemistry
Gene Expression Regulation
/ drug effects
HEK293 Cells
Humans
Membrane Proteins
Myocytes, Cardiac
/ physiology
Propranolol
/ pharmacology
Receptors, Adrenergic, beta-2
/ chemistry
Spectrometry, Fluorescence
/ methods
cardiomyocytes
differentiation
embryonic stem cells
fibroblasts
fluorescence correlation spectroscopy
haplotype
receptor internalization
single-nucleotide polymorphisms
β2-adrenoceptors
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
06
10
2020
revised:
05
01
2021
accepted:
12
01
2021
entrez:
12
3
2021
pubmed:
13
3
2021
medline:
22
7
2021
Statut:
ppublish
Résumé
The importance of cell phenotype in determining the molecular mechanisms underlying β
Identifiants
pubmed: 33710675
doi: 10.1096/fj.202002268R
doi:
Substances chimiques
Adrenergic beta-2 Receptor Agonists
0
Fluorescent Dyes
0
Membrane Proteins
0
Receptors, Adrenergic, beta-2
0
Propranolol
9Y8NXQ24VQ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21398Subventions
Organisme : Medical Research Council
ID : MR/N020081/1
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RG/14/1/30588
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0800006
Pays : United Kingdom
Organisme : RCUK | Medical Research Council (MRC)
ID : MR/N020081/1
Informations de copyright
© 2021 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
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