Responsiveness of human bronchial fibroblasts and epithelial cells from asthmatic and non-asthmatic donors to the transforming growth factor-β
Adult
Aged
Asthma
/ metabolism
Bronchi
/ metabolism
Case-Control Studies
Cell Culture Techniques
Cell Differentiation
Cells, Cultured
Epithelial Cells
/ metabolism
Epithelial-Mesenchymal Transition
/ physiology
Female
Fibroblasts
/ metabolism
Humans
Middle Aged
Myofibroblasts
/ metabolism
Transforming Growth Factor beta1
/ pharmacology
Young Adult
Air-liquid interface
Asthma
Epithelial-mesenchymal trophic unit
Human bronchial (myo)fibroblasts
Human bronchial epithelium
Transforming growth factor-β1
Journal
BMC molecular and cell biology
ISSN: 2661-8850
Titre abrégé: BMC Mol Cell Biol
Pays: England
ID NLM: 101741148
Informations de publication
Date de publication:
12 Mar 2021
12 Mar 2021
Historique:
received:
07
05
2020
accepted:
01
03
2021
entrez:
13
3
2021
pubmed:
14
3
2021
medline:
12
10
2021
Statut:
epublish
Résumé
The asthma-related airway wall remodeling is associated i.a. with a damage of bronchial epithelium and subepithelial fibrosis. Functional interactions between human bronchial epithelial cells and human bronchial fibroblasts are known as the epithelial-mesenchymal trophic unit (EMTU) and are necessary for a proper functioning of lung tissue. However, a high concentration of the transforming growth factor-β Our results show that the asthmatic co-cultures are more sensitive to the TGF-β Our data are the first to demonstrate a protective effect of the human bronchial fibroblasts on the properties of the human bronchial epithelial cells, which suggests that intrinsic properties of not only epithelium but also subepithelial fibroblasts affect a proper condition and function of the EMTU in both normal and asthmatic individuals.
Sections du résumé
BACKGROUND
BACKGROUND
The asthma-related airway wall remodeling is associated i.a. with a damage of bronchial epithelium and subepithelial fibrosis. Functional interactions between human bronchial epithelial cells and human bronchial fibroblasts are known as the epithelial-mesenchymal trophic unit (EMTU) and are necessary for a proper functioning of lung tissue. However, a high concentration of the transforming growth factor-β
RESULTS
RESULTS
Our results show that the asthmatic co-cultures are more sensitive to the TGF-β
CONCLUSIONS
CONCLUSIONS
Our data are the first to demonstrate a protective effect of the human bronchial fibroblasts on the properties of the human bronchial epithelial cells, which suggests that intrinsic properties of not only epithelium but also subepithelial fibroblasts affect a proper condition and function of the EMTU in both normal and asthmatic individuals.
Identifiants
pubmed: 33711932
doi: 10.1186/s12860-021-00356-8
pii: 10.1186/s12860-021-00356-8
pmc: PMC7953709
doi:
Substances chimiques
Transforming Growth Factor beta1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19Subventions
Organisme : Narodowe Centrum Nauki
ID : 2015/17/B/NZ3/02248
Organisme : Narodowe Centrum Nauki
ID : 2019/32/T/NZ3/00405
Références
J Allergy Clin Immunol. 2014 Sep;134(3):663-670.e1
pubmed: 24875618
J Allergy (Cairo). 2012;2012:206109
pubmed: 22988467
Thorax. 2014 Aug;69(8):760-5
pubmed: 24334519
Proc Am Thorac Soc. 2004;1(2):93-8
pubmed: 16113419
Nat Mater. 2015 Oct;14(10):1040-8
pubmed: 26237129
Cell Mol Life Sci. 2018 Nov;75(21):3943-3961
pubmed: 30101406
Exp Lung Res. 2017 Mar;43(2):82-92
pubmed: 28368678
Cell Tissue Res. 2017 Mar;367(3):551-569
pubmed: 28190087
J Allergy Clin Immunol. 1998 Nov;102(5):771-82
pubmed: 9819294
Cytokine. 2018 Feb;102:187-190
pubmed: 28927757
Am J Pathol. 2006 Aug;169(2):405-15
pubmed: 16877343
BMC Cell Biol. 2018 Jun 28;19(1):10
pubmed: 29954317
Int J Mol Sci. 2018 Aug 29;19(9):
pubmed: 30158495
Am J Respir Cell Mol Biol. 1999 Dec;21(6):655-7
pubmed: 10572061
Clin Exp Allergy. 2014 Nov;44(11):1299-313
pubmed: 24661647
Am J Respir Cell Mol Biol. 2014 Aug;51(2):229-41
pubmed: 24588727
Immunol Cell Biol. 2001 Apr;79(2):160-4
pubmed: 11264711
Am J Respir Crit Care Med. 2014 Aug 15;190(4):477-80
pubmed: 25127309
Lab Invest. 2015 Dec;95(12):1418-28
pubmed: 26390052
Lung. 2009 May-Jun;187(3):187-94
pubmed: 19252942
Sci Rep. 2018 Oct 25;8(1):15768
pubmed: 30361541
Fibrogenesis Tissue Repair. 2010 Aug 25;3:15
pubmed: 20738867
Proc Am Thorac Soc. 2009 Dec;6(8):678-82
pubmed: 20008875
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13132-13137
pubmed: 27799535
Allergol Int. 2008 Mar;57(1):1-10
pubmed: 18209502
Am J Respir Cell Mol Biol. 2017 Jul;57(1):100-110
pubmed: 28245135
J Clin Med. 2017 Nov 29;6(12):
pubmed: 29186064
Tissue Barriers. 2016 Jun 28;4(3):e1206378
pubmed: 27583193
Thorax. 2009 Dec;64(12):1053-61
pubmed: 19850962
Allergy Asthma Immunol Res. 2013 Nov;5(6):343-7
pubmed: 24179679
Sci Rep. 2020 Jan 22;10(1):966
pubmed: 31969610
Am J Respir Cell Mol Biol. 2018 Jul;59(1):104-113
pubmed: 29394092
J Allergy Clin Immunol. 2017 Jan;139(1):93-103
pubmed: 27312821
Respir Med. 2011 Oct;105(10):1467-75
pubmed: 21802932
J Allergy Clin Immunol. 2011 Sep;128(3):451-62; quiz 463-4
pubmed: 21636119
Am J Respir Crit Care Med. 2001 Feb;163(2):517-23
pubmed: 11179133
BMC Cell Biol. 2016 Mar 01;17:7
pubmed: 26932661
J Lab Autom. 2015 Apr;20(2):107-26
pubmed: 25586998
Am J Respir Cell Mol Biol. 2008 May;38(5):517-23
pubmed: 18063839
Sci Rep. 2020 Oct 5;10(1):16492
pubmed: 33020537
Respir Res. 2017 Nov 22;18(1):195
pubmed: 29166920
J Allergy (Cairo). 2012;2012:943982
pubmed: 22287976
Respir Res. 2013 Nov 01;14:118
pubmed: 24283210
Med Sci Monit. 2009 Jul;15(7):BR194-201
pubmed: 19564819
Lab Invest. 2014 Sep;94(9):991-1002
pubmed: 24955895
Am J Respir Cell Mol Biol. 2011 Nov;45(5):1090-100
pubmed: 21642587
J Allergy Clin Immunol. 2011 Sep;128(3):549-56.e1-12
pubmed: 21752437
Am J Respir Crit Care Med. 2009 Jul 15;180(2):122-33
pubmed: 19406982
Respir Res. 2018 Aug 2;19(1):145
pubmed: 30068332
COPD. 2009 Dec;6(6):421-31
pubmed: 19938964
PLoS One. 2015 Feb 13;10(2):e0116840
pubmed: 25679502
Pol Arch Med Wewn. 2012;122(7-8):326-32
pubmed: 22814516
Respir Res. 2015 Feb 13;16:21
pubmed: 25849331
Respir Res. 2015 Jun 18;16:72
pubmed: 26081431