Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 03 2021
12 03 2021
Historique:
received:
27
03
2020
accepted:
12
02
2021
entrez:
13
3
2021
pubmed:
14
3
2021
medline:
2
4
2021
Statut:
epublish
Résumé
Many bacterial pathogens use a type III secretion system (T3SS) to manipulate host cells. Protein secretion by the T3SS injectisome is activated upon contact to any host cell, and it has been unclear how premature secretion is prevented during infection. Here we report that in the gastrointestinal pathogens Yersinia enterocolitica and Shigella flexneri, cytosolic injectisome components are temporarily released from the proximal interface of the injectisome at low external pH, preventing protein secretion in acidic environments, such as the stomach. We show that in Yersinia enterocolitica, low external pH is detected in the periplasm and leads to a partial dissociation of the inner membrane injectisome component SctD, which in turn causes the dissociation of the cytosolic T3SS components. This effect is reversed upon restoration of neutral pH, allowing a fast activation of the T3SS at the native target regions within the host. These findings indicate that the cytosolic components form an adaptive regulatory interface, which regulates T3SS activity in response to environmental conditions.
Identifiants
pubmed: 33712575
doi: 10.1038/s41467-021-21863-4
pii: 10.1038/s41467-021-21863-4
pmc: PMC7954860
doi:
Substances chimiques
Bacterial Proteins
0
Type III Secretion Systems
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1625Commentaires et corrections
Type : ErratumIn
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