Promoter-specific expression of the imprinted IGF2 gene in bovine oocytes and pre-implantation embryos.
IGF2
IVP embryos
cattle
expression
imprinting
promoter
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
26
01
2021
accepted:
10
03
2021
pubmed:
14
3
2021
medline:
21
10
2021
entrez:
13
3
2021
Statut:
ppublish
Résumé
The bovine IGF2 locus is a genomic region with alternative transcripts controlled by five promoters (P0, P1, P2, P3 and P4). As transcriptional regulation can affect messenger RNA (mRNA) stability and translation, and thus, subsequent biological effects, this study evaluated the bovine IGF2 promoter-specific expression patterns in oocytes and pre-implantation embryos produced in vitro by our standard IVP procedures. Immature and matured oocytes, and pre-implantation embryos at the 1-, 2-, 4-, 8- and 16-cell, and at early morula, compact morula, blastocyst and expanded blastocyst stages were collected in three pools of five structures per stage, in four replicates. Total RNA was extracted and subjected to RT-qPCR, using four sets of IGF2 promoter-specific primers covering transcripts driven by promoters P0/P1, P2, P3 and P4, with fragments sequenced for confirmation. Expression of P2- and P4-derived transcripts showed an initial peak between immature (P4) or matured (P2/P4) oocytes and 2-cell embryos, gradually falling until embryo genome activation (EGA), rising again at compaction and cavitation. P0/P1-derived transcripts were identified after EGA, during compaction, whereas P3 activity was not detected at any stage. Our findings suggest that P0/P1 and P2 likely have secondary roles during early stages, whereas P3 may be more relevant later in development. P4 seems to be the main pathway for bovine IGF2 expression during oocyte maturation and embryo development and, therefore, the main target to influence IVP in modulation of embryo growth and in studies in developmental biology.
Substances chimiques
RNA, Messenger
0
Insulin-Like Growth Factor II
67763-97-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
857-863Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 407.280/2013-8
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 449.509/2014-1
Informations de copyright
© 2021 Wiley-VCH GmbH.
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