Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry.

Animals Drosophila / genetics Drosophila Proteins / genetics Drosophila melanogaster / genetics Hemocytes / metabolism Janus Kinases / genetics Larva / metabolism
Drosophila Hemocyte Innate immunity Mass cytometry Single-cell analysis

Journal

Genomics, proteomics & bioinformatics
ISSN: 2210-3244
Titre abrégé: Genomics Proteomics Bioinformatics
Pays: China
ID NLM: 101197608

Informations de publication

Date de publication:
04 2021
Historique:
received: 14 11 2019
revised: 11 06 2020
accepted: 28 06 2020
pubmed: 14 3 2021
medline: 24 3 2022
entrez: 13 3 2021
Statut: ppublish

Résumé

Single-cell mass cytometry (SCMC) combines features of traditional flow cytometry (i.e., fluorescence-activated cell sorting) with mass spectrometry, making it possible to measure several parameters at the single-cell level for a complex analysis of biological regulatory mechanisms. In this study, weoptimizedSCMC to analyze hemocytes of the Drosophila innate immune system. We used metal-conjugated antibodies (against cell surface antigens H2, H3, H18, L1, L4, and P1, and intracellular antigens 3A5 and L2) and anti-IgM (against cell surface antigen L6) to detect the levels of antigens, while anti-GFP was used to detect crystal cells in the immune-induced samples. We investigated the antigen expression profile of single cells and hemocyte populations in naive states, in immune-induced states, in tumorous mutants bearing a driver mutation in the Drosophila homologue of Janus kinase (hop

Identifiants

DOI: 10.1016/j.gpb.2020.06.022 PMID: 33713850 PMC: PMC8602394
pubmed: 33713850
pii: S1672-0229(21)00055-3
doi: 10.1016/j.gpb.2020.06.022
pmc: PMC8602394
pii:
doi:

Substances chimiques

Drosophila Proteins 0
Janus Kinases EC 2.7.10.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

243-252

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Références

Nat Biotechnol. 2013 Jun;31(6):545-52
pubmed: 23685480
EMBO J. 2020 Jun 17;39(12):e104486
pubmed: 32162708
Wiley Interdiscip Rev Dev Biol. 2019 Sep;8(5):e344
pubmed: 30993906
Infect Immun. 2009 Jul;77(7):3044-55
pubmed: 19364838
Eur J Immunol. 1976 Jul;6(7):511-9
pubmed: 825377
Front Neurosci. 2018 Jan 04;11:732
pubmed: 29354028
Ann Rheum Dis. 2020 Nov;79(11):1522-1524
pubmed: 32424030
Immunol Lett. 2009 Sep 22;126(1-2):83-4
pubmed: 19695290
Genes (Basel). 2019 Mar 05;10(3):
pubmed: 30841641
Trends Immunol. 2020 Mar;41(3):190-199
pubmed: 32035764
PLoS One. 2017 May 17;12(5):e0176689
pubmed: 28520752
Front Genet. 2019 Mar 01;10:51
pubmed: 30881374
EMBO J. 1995 Jun 15;14(12):2857-65
pubmed: 7796812
Nat Commun. 2020 Sep 8;11(1):4483
pubmed: 32900993
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2622-7
pubmed: 12598653
Mol Immunol. 2010 Jul;47(11-12):1997-2004
pubmed: 20483458
J Immunol. 2007 Apr 15;178(8):4711-6
pubmed: 17404248
Eur J Cell Biol. 1995 Dec;68(4):450-6
pubmed: 8690025
BMC Dev Biol. 2010 Jun 11;10:65
pubmed: 20540764
Scand J Immunol. 1991 Nov;34(5):531-7
pubmed: 1835124
Curr Biol. 2007 Apr 3;17(7):649-54
pubmed: 17363253
Chem Immunol Allergy. 2005;86:1-21
pubmed: 15976485
Cells. 2019 Sep 16;8(9):
pubmed: 31527554
Cytometry B Clin Cytom. 2019 Jan;96(1):46-56
pubmed: 30426661
Parasitology. 1996 Jan;112 ( Pt 1):135-42
pubmed: 8587797
Acta Biol Hung. 2007;58 Suppl:95-111
pubmed: 18297797
PLoS One. 2010 Nov 19;5(11):e14051
pubmed: 21124962
Elife. 2020 May 12;9:
pubmed: 32396065
Front Physiol. 2019 May 15;10:603
pubmed: 31156469
Int J Mol Sci. 2019 Dec 25;21(1):
pubmed: 31881770
Dev Biol. 2003 Dec 15;264(2):582-91
pubmed: 14651939
Development. 1995 Dec;121(12):4149-60
pubmed: 8575315
Genesis. 2009 Nov;47(11):771-4
pubmed: 19830816
Dev Biol. 2001 Feb 15;230(2):243-57
pubmed: 11161576
Genesis. 2012 Jan;50(1):41-9
pubmed: 21809435
PLoS Pathog. 2016 Jul 14;12(7):e1005746
pubmed: 27414410
PLoS Pathog. 2014 May 01;10(5):e1004067
pubmed: 24788090
Nat Commun. 2017 May 26;8:15327
pubmed: 28548102
Dis Model Mech. 2017 Mar 1;10(3):271-281
pubmed: 28250052
Dev Comp Immunol. 2014 Jan;42(1):47-56
pubmed: 23800719
Science. 2000 Apr 7;288(5463):146-9
pubmed: 10753120
FEBS J. 2019 Jul;286(14):2670-2691
pubmed: 30993828
Dev Biol. 1994 May;163(1):98-111
pubmed: 8174791
Methods. 2014 Jun 15;68(1):242-51
pubmed: 24613936

Auteurs

József Á Balog (JÁ)

Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary; University of Szeged, Ph.D. School in Biology, Szeged H-6726, Hungary.

Viktor Honti (V)

Immunology Unit, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary.

Éva Kurucz (É)

Immunology Unit, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary.

Beáta Kari (B)

Immunology Unit, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary.

László G Puskás (LG)

Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary.

István Andó (I)

Immunology Unit, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary. Electronic address: ando@brc.hu.

Gábor J Szebeni (GJ)

Laboratory of Functional Genomics, Institute of Genetics, Biological Research Centre, Szeged H-6726, Hungary; Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged H-6726, Hungary. Electronic address: szebeni.gabor@brc.hu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Immunoprofiling of Drosophila Hemocytes by...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Immunoprofiling of Drosophila Hemocytes by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Immunoprofiling of Drosophila Hemocytes by...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › Immunoprofiling of Drosophila Hemocytes by...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Hémocytes › Immunoprofiling of Drosophila Hemocytes by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-tyrosine kinases › Janus kinases › Immunoprofiling of Drosophila Hemocytes by...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › Immunoprofiling of Drosophila Hemocytes by...