ELISA detection of anti-desmoglein 1 and anti-desmoglein 3 and indirect immunofluorescence in oral pemphigus: A retrospective study.
ELISA
indirect immunofluorescence
oral vesiculo-bullous disease
pemphigus
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
revised:
03
03
2021
received:
24
11
2020
accepted:
07
03
2021
pubmed:
16
3
2021
medline:
29
4
2022
entrez:
15
3
2021
Statut:
ppublish
Résumé
The aim of this study was to test the efficacy of autoantibodies to desmoglein 1 and desmoglein 3 detected by ELISA and indirect immunofluorescence in the diagnosis of oral pemphigus and to correlate the antibody titres with the severity of the disease. We report a retrospective cohort study of 22 patients with oral pemphigus and 64 controls from a single tertiary centre. Data about histopathological examination, direct immunofluorescence, indirect immunofluorescence and ELISA were analysed. Global validation of ELISA and IIF both alone and combined was established by calculating sensitivity, specificity, accuracy and both positive predictive value and negative predictive value. The relationship between Oral Disease Severity Score values and ELISA titres was analysed using Pearson's coefficient. The best diagnostic performance was observed for anti-desmoglein 3 ELISA. The sensitivity was 75% and specificity 100% and positive predictive value and negative predictive value were 92.5% and accuracy 93.9%. The level of agreement with histopathology + direct immunofluorescence was substantial (k = .758). Anti-desmoglein 3 titres showed a significant correlation with Oral Disease Severity Score (p < .05). Serological tests are commonly employed during clinical practice as adjunctive tools. Anti-desmoglein 3 ELISA should be considered as a first-instance diagnostic test for oral pemphigus early detection.
Substances chimiques
Autoantibodies
0
Desmoglein 1
0
Desmoglein 3
0
Types de publication
Journal Article
Langues
eng
Pagination
1149-1156Informations de copyright
© 2021 Wiley Periodicals LLC.
Références
Abasq, C., Mouquet, H., Gilbert, D., Tron, F., Grassi, V., Musette, P., & Joly, P. (2009). ELISA testing of anti-desmoglein 1 and 3 antibodies in the management of pemphigus. Archives of Dermatology, 145(5), 529-535. https://doi.org/10.1001/archdermatol.2009.9
Amagai, M., Tsunoda, K., Zillikens, D., Nagai, T., & Nishikawa, T. (1999). The clinical phenotype of pemphigus is defined by the anti-desmoglein autoantibody profile. Journal of American Academy of Dermatology, 40(2 Pt 1), 167-170. https://doi.org/10.1016/s0190-9622(99)70183-0
Amber, K. T., Valdebran, M., & Grando, S. A. (2018). Non-desmoglein ANTIBODIES IN PATIENTS WITH PEMPHIGUS VULGARIS. Frontiers in Immunology, 9, 1190. https://doi.org/10.3389/fimmu.2018.01190
Bascones-Martinez, A., Munoz-Corcurera, M., Bascones-Illundain, C., & Esparza-Gómez, G. (2010). Oral manifestations of pemphigus vulgaris: Clinical presentation, differential diagnosis and management. Journal of Clinical and Experimental Dermatology Research, 1, 112. https://doi.org/10.4172/2155-9554.1000112
Buonavoglia, A., Leone, P., Dammacco, R., Di Lernia, G., Petruzzi, M., Bonamonte, D., Vacca, A., Racanelli, V., & Dammacco, F. (2019). Pemphigus and mucous membrane pemphigoid: An update from diagnosis to therapy. Autoimmunity Review, 18(4), 349-358. https://doi.org/10.1016/j.autrev.2019.02.005
Chang, J. Y., Chiang, C. P., Hsiao, C. K., & Sun, A. (2009). Significantly higher frequencies of presence of serum autoantibodies in Chinese patients with oral lichen planus. Journal of Oral Pathology & Medicine, 38(1), 48-54. https://doi.org/10.1111/j.1600-0714.2008.00686.x
Cholera, M., & Chainani-Wu, N. (2016). Management of pemphigus vulgaris. Advances in Therapy, 33(6), 910-958. https://doi.org/10.1007/s12325-016-0343-4
Daltaban, Ö., Özçentik, A., Akman Karakaş, A., Üstün, K., Hatipoğlu, M., & Uzun, S. (2020). Clinical presentation and diagnostic delay in pemphigus vulgaris: A prospective study from Turkey. Journal of Oral Pathology & Medicine, 49(7), 681-686. https://doi.org/10.1111/jop.13052
Delavarian, Z., Layegh, P., Pakfetrat, A., Zarghi, N., Khorashadizadeh, M., & Ghazi, A. (2020). Evaluation of desmoglein 1 and 3 autoantibodies in pemphigus vulgaris: Correlation with disease severity. Journal of Clinical and Experimental Dentistry, 12(5), e440-e445. https://doi.org/10.4317/jced.56289
Di Lernia, V., Casanova, D. M., Goldust, M., & Ricci, C. (2020). Pemphigus vulgaris and bullous pemphigoid: Update on diagnosis and treatment. Dermatology Practical & Conceptual, 10(3), e2020050. https://doi.org/10.5826/dpc.1003a50
Didona, D., & Di Zenzo, G. (2018). Humoral epitope spreading in autoimmune bullous diseases. Frontiers in Immunology, 9, 779. https://doi.org/10.3389/fimmu.2018.00779
Endo, H., Rees, T. D., Allen, E. P., Kuyama, K., Aoki, S., Yamamoto, H., & Ito, T. (2014). A stab-and-roll biopsy technique to maintain gingival epithelium for desquamative gingivitis. Journal of Periodontology, 85(6), 802-809. https://doi.org/10.1902/jop.2014.130428
Hallaji, Z., Mortazavi, H., Lajevardi, V., Tamizifar, B., AmirZargar, A., Daneshpazhooh, M., & Chams-Davatchi, C. (2010). Serum and salivary desmoglein 1 and 3 enzyme-linked immunosorbent assay in pemphigus vulgaris: Correlation with phenotype and severity. Journal of the European Academy of Dermatology and Venereology, 24(3), 275-280. https://doi.org/10.1111/j.1468-3083.2009.03408.x
Harman, K. E., Seed, P. T., Gratian, M. J., Bhogal, B. S., Challacombe, S. J., & Black, M. M. (2001). The severity of cutaneous and oral pemphigus is related to desmoglein 1 and 3 antibody levels. TheBritish Journal of Dermatology, 144(4), 775-780. https://doi.org/10.1046/j.1365-2133.2001.04132.x
Herrero-González, J. E., Iranzo, P., Benítez, D., Lozano, F., Herrero, C., & Mascaró, J. M. (2010). Correlation of immunological profile with phenotype and disease outcome in pemphigus. Acta Dermato-Venereologica, 90, 401-405. https://doi.org/10.2340/00015555-0868
Kridin, K., & Bergman, R. (2018). The usefulness of indirect immunofluorescence in pemphigus and the natural history of patients with initial false-positive results: A retrospective cohort study. Frontiers in Medicine, 5, 266. https://doi.org/10.3389/fmed.2018.00266
Landis, J. R., & Koch, G. G. (1977). The measurement of observer agreement for categorical data. Biometrics, 33(1), 159-174. https://doi.org/10.2307/2529310
Li, Z., Zhang, J., Xu, H., Jin, P., Feng, S., & Wang, B. (2015). Correlation of conventional and conformational anti-desmoglein antibodies with phenotypes and disease activities in patients with pemphigus vulgaris. Acta Dermato-Venereologica, 95(4), 462-465. https://doi.org/10.2340/00015555-1961
Ministero della Salute Italiano. (2021, January 20). Retrieved from www.salute.gov.it
Mortazavi, H., Kiavash, K., Esmaili, N., & Chams-Davatchi, C. (2008). Correlation of pemphigus vulgaris antibody titers by indirect immunofluorescence with activity of disease based on pemphigus area and activity score (PAAS). Acta Medica Iranica, 46, 239-244.
Mortazavi, H., Shahdi, M., Amirzargar, A. A., Naraghi, Z. S., Valikhani, M., Daneshpazhooh, M., Vasheghani-Farahani, A., Sedaghat, M., & Chams-Davatchi, C. (2009). Desmoglein ELISA in the diagnosis of pemphigus and its correlation with the severity of pemphigus vulgaris. Iranian Journal of Allergy, Asthma, and Immunology, 8(1), 53-56.
Muramatsu, K., Nishie, W., Natsuga, K., Fujita, Y., Iwata, H., Yamada, T., Yamashita, E., Asaka, T., & Shimizu, H. (2016). Two cases of erosive oral lichen planus with autoantibodies to desmoglein 3. The Journal of Dermatology, 43(11), 1350-1353. https://doi.org/10.1111/1346-8138.13493
Murrell, D. F., Peña, S., Joly, P., Marinovic, B., Hashimoto, T., Diaz, L. A., Sinha, A. A., Payne, A. S., Daneshpazhooh, M., Eming, R., Jonkman, M. F., Mimouni, D., Borradori, L., Kim, S.-C., Yamagami, J., Lehman, J. S., Saleh, M. A., Culton, D. A., Czernik, A., … Werth, V. P. (2020). Diagnosis and management of pemphigus: Recommendations of an international panel of experts. Journal of the American Academy of Dermatology, 82(3), 575-585. https://doi.org/10.1016/j.jaad.2018.02.021
Ormond, M., McParland, H., Donaldson, A., Andiappan, M., Cook, R. J., Escudier, M., Hullah, E., Higham, J., McMillan, R., Taylor, J., Shirlaw, P. J., Challacombe, S. J., & Setterfield, J. F. (2018). An Oral Disease Severity Score validated for use in oral pemphigus vulgaris. The British Journal of Dermatology, 179(4), 872-881. https://doi.org/10.1111/bjd.16265
Petruzzi, M., Tampoia, M., Serpico, R., Lauritano, D., Lajolo, C., Lucchese, A., & Della Vella, F. (2021). Evaluation of BP180-NC16A ELISA in exclusive oral pemphigoid diagnosis. A Comparative Study. Oral Diseases, 27(3), 525-531. https://doi.org/10.1111/odi.13574
Pollmann, R., Schmidt, T., Eming, R., & Hertl, M. (2018). Pemphigus: A comprehensive review on pathogenesis, clinical presentation and novel therapeutic approaches. Clinical Reviews in Allergy & Immunology, 54(1), 1-25. https://doi.org/10.1007/s12016-017-8662-z
Russo, I., Saponeri, A., Michelotto, A., & Alaibac, M. (2017). Salivary samples for the diagnosis of pemphigus vulgaris using the BIOCHIP approach: A pilot study. In Vivo (Athens, Greece), 31(1), 97-99. https://doi.org/10.21873/invivo.11030
Saschenbrecker, S., Karl, I., Komorowski, L., Probst, C., Dähnrich, C., Fechner, K., Stöcker, W., & Schlumberger, W. (2019). Serological diagnosis of autoimmune bullous skin diseases. Frontiers in Immunology, 20(10), 1974. https://doi.org/10.3389/fimmu.2019.01974
Schmidt, E., Kasperkiewicz, M., & Joly, P. (2019). Pemphigus. Lancet, 394(10201), 882-894. https://doi.org/10.1016/S0140-6736(19)31778-7
Schmidt, E., & Zillikens, D. (2010). Modern diagnosis of autoimmune blistering skin diseases. Autoimmunity Reviews, 10(2), 84-89. https://doi.org/10.1016/j.autrev.2010.08.007
Schmidt, E., & Zillikens, D. (2011). The diagnosis and treatment of autoimmune blistering skin diseases. Deutsches Ärzteblatt. International, 108, 399-III. https://doi.org/10.3238/arztebl.2011.0399
Schultz, B., Latour, E., & Fett, N. (2019). Quality of life remains poor for patients with pemphigus vulgaris despite targeted therapies. The British Journal of Dermatology, 181(5), 1101-1103. https://doi.org/10.1111/bjd.18167
Sharma, V. K., Prasad, H. R., Khandpur, S., & Kumar, A. (2006). Evaluation of desmoglein enzyme-linked immunosorbent assay (ELISA) in Indian patients with pemphigus vulgaris. International Journal of Dermatology, 45(5), 518-522. https://doi.org/10.1111/j.1365-4632.2006.02593.x
Sultan, A. S., Villa, A., Saavedra, A. P., Treister, N. S., & Woo, S. B. (2017). Oral mucous membrane pemphigoid and pemphigus vulgaris-a retrospective two-center cohort study. Oral Diseases, 23(4), 498-504. https://doi.org/10.1111/odi.12639
Tampoia, M., Giavarina, D., Di Giorgio, C., & Bizzaro, N. (2012). Diagnostic accuracy of enzyme-linked immunosorbent assays (ELISA) to detect anti-skin autoantibodies in autoimmune blistering skin diseases: A systematic review and meta-analysis. Autoimmunity Reviews, 12(2), 121-126. https://doi.org/10.1016/j.autrev.2012.07.006
van Beek, N., Dähnrich, C., Johannsen, N., Lemcke, S., Goletz, S., Hübner, F., Di Zenzo, G., Dmochowski, M., Drenovska, K., Geller, S., Horn, M., Kowalewski, C., Medenica, L., Murrell, D. F., Patsatsi, A., Uzun, S., Vassileva, S., Zillikens, D., Schlumberger, W., & Schmidt, E. (2017). Prospective studies on the routine use of a novel multivariant enzyme-linked immunosorbent assay for the diagnosis of autoimmune bullous diseases. Journal of the American Academy of Dermatology, 76(5), 889-894.e5. https://doi.org/10.1016/j.jaad.2016.11.002
Walton, R., Robinson, M., & Carrozzo, M. (2020). A service evaluation of the diagnostic testing for mucous membrane pemphigoid in a UK oral medicine unit. Journal of Oral Pathology & Medicine, 49(7), 687-692. https://doi.org/10.1111/jop.13054
Weiss, D., Ristl, R., Griss, J., Bangert, C., Foedinger, D., Stingl, G., & Brunner, P. M. (2015). Autoantibody levels and clinical disease severity in patients with pemphigus: Comparison of aggregated anti-desmoglein ELISA values and indirect immunofluorescence titres. Acta Dermato-Venereologica, 95(5), 559-564. https://doi.org/10.2340/00015555-2023
Zhou, T., Fang, S., Li, C., & Hua, H. (2016). Comparative study of indirect immunofluorescence, enzyme-linked immunosorbent assay, and the Tzanck smear test for the diagnosis of pemphigus. Journal of Oral Pathology & Medicine, 45(10), 786-790. https://doi.org/10.1111/jop.12439