The red blood cell as a novel regulator of human B-cell activation.
Aged
Aged, 80 and over
Anemia, Hemolytic, Autoimmune
/ immunology
Antigens, CD
/ metabolism
Antigens, Differentiation, T-Lymphocyte
/ metabolism
B-Lymphocytes
/ immunology
CD40 Antigens
/ metabolism
Cell Proliferation
Cells, Cultured
Erythrocytes
/ immunology
Female
Humans
Immunoglobulin M
/ metabolism
Lectins, C-Type
/ metabolism
Lymphocyte Activation
Male
Middle Aged
Sialic Acid Binding Ig-like Lectin 2
/ metabolism
Sialic Acids
/ metabolism
Up-Regulation
B cell
CD22
human
immune regulation
red blood cell
sialic acid
Journal
Immunology
ISSN: 1365-2567
Titre abrégé: Immunology
Pays: England
ID NLM: 0374672
Informations de publication
Date de publication:
08 2021
08 2021
Historique:
revised:
12
02
2021
received:
19
05
2020
accepted:
01
03
2021
pubmed:
18
3
2021
medline:
5
10
2021
entrez:
17
3
2021
Statut:
ppublish
Résumé
Non-immune cells are increasingly recognized as important in regulating immunity, but the role of red blood cells (RBC) remains relatively unexplored, despite their abundance in the circulation and a cell surface rich in potential ligands. Here, we determine whether RBC influence the activation state of human B cells. Separation of RBC from peripheral blood mononuclear cells increased B-cell expression of HLA-DR/DP/DQ, whilst reconstitution reduced the levels of B-cell activation markers HLA-DR/DP/DQ, CD86, CD69 and CD40, as well as decreasing proliferative responses and IgM secretion. Inhibition of B cells required contact with RBC and was abrogated by either removal of sialic acids from RBC or blocking the corresponding lectin receptor CD22 on B cells. Chronic lymphocytic leukaemia B cells express low levels of CD22 and were less susceptible to inhibition by RBC, which may contribute to their activated phenotype. Taken together, the results identify a novel mechanism that may suppress inappropriate responsiveness of healthy B cells whilst circulating in the bloodstream.
Identifiants
pubmed: 33728669
doi: 10.1111/imm.13327
pmc: PMC8274151
doi:
Substances chimiques
Antigens, CD
0
Antigens, Differentiation, T-Lymphocyte
0
CD40 Antigens
0
CD69 antigen
0
Immunoglobulin M
0
Lectins, C-Type
0
Sialic Acid Binding Ig-like Lectin 2
0
Sialic Acids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
436-447Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 094847
Pays : United Kingdom
Informations de copyright
© 2021 The Authors. Immunology published by John Wiley & Sons Ltd.
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