Tuning the Phenotype of Cartilage Tissue Mimics by Varying Spheroid Maturation and Methacrylamide-Modified Gelatin Hydrogel Characteristics.
bioink
bioprinting
cartilages
chondrocytes
methacrylamide-modified gelatin
self-assemblies
spheroids
Journal
Macromolecular bioscience
ISSN: 1616-5195
Titre abrégé: Macromol Biosci
Pays: Germany
ID NLM: 101135941
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
27
01
2021
pubmed:
18
3
2021
medline:
21
1
2022
entrez:
17
3
2021
Statut:
ppublish
Résumé
In hybrid bioprinting of cartilage tissue constructs, spheroids are used as cellular building blocks and combined with biomaterials for dispensing. However, biomaterial intrinsic cues can deeply affect cell fate and to date, the influence of hydrogel encapsulation on spheroid viability and phenotype has received limited attention. This study assesses this need and unravels 1) how the phenotype of spheroid-laden constructs can be tuned through adjusting the hydrogel physico-chemical properties and 2) if the spheroid maturation stage prior to encapsulation is a determining factor for the construct phenotype. Articular chondrocyte spheroids with a cartilage specific extracellular matrix (ECM) are generated and different maturation stages, early-, mid-, and late-stage (3, 7, and 14 days, respectively), are harvested and encapsulated in 10, 15, or 20 w/v% methacrylamide-modified gelatin (gelMA) for 14 days. The encapsulation of immature spheroids do not lead to a cartilage-like ECM production but when more mature mid- or late-stage spheroids are combined with a certain concentration of gelMA, a fibrocartilage-like as well as a hyaline cartilage-like phenotype can be induced. As a proof of concept, late-stage spheroids are bioprinted using a 10 w/v% gelMA-Irgacure 2959 solution with the aim to test the processing potential of the spheroid-laden bioink.
Identifiants
pubmed: 33729714
doi: 10.1002/mabi.202000401
doi:
Substances chimiques
Acrylamides
0
Hydrogels
0
Gelatin
9000-70-8
methacrylamide
K67NG89J77
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000401Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
A. J. S. Fox, A. Bedi, S. A. Rodeo, Sports Health 2012, 4, 340.
A. J. S. Fox, A. Bedi, S. A. Rodeo, Sports Health 2009, 1, 461.
R. Davies, N. Kuiper, Bioengineering 2019, 6, 22.
G. Knutsen, J. O. Drogset, L. Engebretsen, T. Grøntvedt, V. Isaksen, T. C. Ludvigsen, S. Roberts, E. Solheim, T. Strand, O. Johansen, J. Bone Jt. Surg. 2007, 89, 2105.
L. De Moor, E. Beyls, H. Declercq, Ann. Biomed. Eng. 2020, 48, 298.
M. M. J. Caron, P. J. Emans, M. M. E. Coolsen, L. Voss, D. A. M. Surtel, A. Cremers, L. W. van Rhijn, T. J. M. Welting, Osteoarthritis and Cartilage 2012, 20, 1170.
B. K. Babur, P. Ghanavi, P. Levett, W. B. Lott, T. Klein, J. J. Cooper-White, R. Crawford, M. R. Doran, PLoS One 2013, 8, e58865.
U. Freymann, M. Endres, U. Goldmann, M. Sittinger, C. Kaps, Osteoarthritis and Cartilage 2013, 21, 773.
L. S. M. Teixeira, J. C. H. Leijten, J. Sobral, R. Jin, A. A. van Apeldoorn, J. Feijen, C. van Blitterswijk, P. J. Dijkstra, M. Karperien, Eur. Cells Mater. 2012, 23, 387.
E. Berneel, C. Philips, H. Declercq, R. Cornelissen, Cells Tissues Organs 2016, 202, 369.
G.-K. Tan, D. L. M. Dinnes, P. T. Myers, J. J. Cooper-White, Biomaterials 2011, 32, 5600.
L. Zhang, P. Su, C. Xu, J. Yang, W. Yu, D. Huang, Biotechnol. Lett. 2010, 32, 1339.
J. W. Nichol, A. Khademhosseini, Soft Matter 2009, 5, 1312.
X. Armoiry, E. Cummins, M. Connock, A. Metcalfe, P. Royle, R. Johnston, J. Rodrigues, N. Waugh, H. Mistry, Pharmacoeconomics 2019, 37, 879.
U. Anderer, J. Libera, J. Bone Miner. Res. 2002, 17, 1420.
N. Moldovan, L. Maldovan, M. Raghunath, Int. J. Bioprint. 2018, 5, 167.
B. R. Freedman, D. J. Mooney, Adv. Mater. 2019, 31, 1806695.
A. C. Daly, S. E. Critchley, E. M. Rencsok, D. J. Kelly, Biofabrication 2016, 8, 045002.
V. H. M. Mouser, R. Levato, A. Mensinga, W. J. A. Dhert, D. Gawlitta, J. Malda, Connect. Tissue Res. 2018, 61, 137.
M. Costantini, J. Idaszek, K. Szöke, J. Jaroszewicz, M. Dentini, A. Barbetta, J. E. Brinchmann, W. Święszkowski, Biofabrication 2016, 8, 035002.
A. I. Van Den Bulcke, B. Bogdanov, N. De Rooze, E. H. Schacht, M. Cornelissen, H. Berghmans, Biomacromolecules 2000, 1, 31.
I. Pepelanova, K. Kruppa, T. Scheper, A. Lavrentieva, Bioengineering 2018, 5, 55.
D. Loessner, C. Meinert, E. Kaemmerer, L. C. Martine, K. Yue, P. A. Levett, T. J. Klein, F. P. W. Melchels, A. Khademhosseini, D. W. Hutmacher, Nat. Protoc. 2016, 11, 727.
S. Pahoff, C. Meinert, O. Bas, L. Nguyen, T. J. Klein, D. W. Hutmacher, J. Mater. Chem. B 2019, 7, 1761.
A. D. Rouillard, C. M. Berglund, J. Y. Lee, W. J. Polacheck, Y. Tsui, L. J. Bonassar, B. J. Kirby, Tissue Eng., Part C 2011, 17, 173.
T. Billiet, E. Gevaert, T. De Schryver, M. Cornelissen, P. Dubruel, Biomaterials 2014, 35, 49.
L. Tytgat, S. Baudis, H. Ottevaere, R. Liska, H. Thienpont, P. Dubruel, S. Van Vlierberghe, in 3D Print. Biofabrication (Eds: A. Ovsianikov, J. Yoo, V. Mironov), Springer International Publishing, New York 2018, pp. 353-396.
X. Hu, L. Ma, C. Wang, C. Gao, Macromol. Biosci. 2009, 9, 1194.
W. Schuurman, P. A. Levett, M. W. Pot, P. R. van Weeren, W. J. A. A. Dhert, D. W. Hutmacher, F. P. W. W. Melchels, T. J. Klein, J. Malda, Macromol. Biosci. 2013, 13, 551.
S. A. Ferreira, P. A. Faull, A. J. Seymour, T. T. L. Yu, S. Loaiza, H. W. Auner, A. P. Snijders, E. Gentleman, Biomaterials 2018, 176, 13.
A. K. Dewan, M. A. Gibson, J. H. Elisseeff, M. E. Trice, Biomed Res. Int. 2014, 2014, 272481.
Z. Deng, J. Jin, J. Zhao, H. Xu, Stem Cells Int. 2016, 2016, 9201492.
C. Onofrillo, S. Duchi, C. D. O'Connell, R. Blanchard, A. J. O'Connor, M. Scott, G. G. Wallace, P. F. M. Choong, C. Di Bella, Biofabrication 2018, 10, 045006.
C. R. Chu, M. Szczodry, S. Bruno, Tissue Eng., Part B 2010, 16, 105.
D. Deponti, A. Di Giancamillo, C. Scotti, G. M. Peretti, I. Martin, J. Tissue Eng. Regener. Med. 2015, 9, 512.
L. De Moor, S. Fernandez, C. Vercruysse, L. Tytgat, M. Asadian, N. De Geyter, S. Van Vlierberghe, P. Dubruel, H. Declercq, Front. Bioeng. Biotechnol. 2020, 8, 484.
C. Madeira, A. Santhagunam, J. B. Salgueiro, J. M. S. Cabral, Trends Biotechnol. 2015, 33, 35.
V. Mironov, R. P. Visconti, V. Kasyanov, G. Forgacs, C. J. Drake, R. R. Markwald, Biomaterials 2009, 30, 2164.
I. Martinez, J. Elvenes, R. Olsen, K. Bertheussen, O. Johansen, Cell Transplant. 2008, 17, 987.
A. Ruedel, S. Hofmeister, A.-K. Bosserhoff, Int. J. Clin. Exp. Pathol. 2013, 6, 3042.
K. P. Quinn, E. Bellas, N. Fourligas, K. Lee, D. L. Kaplan, I. Georgakoudi, Biomaterials 2012, 33, 5341.
G. N. Hall, L. F. Mendes, C. Gklava, L. Geris, F. P. Luyten, I. Papantoniou, Adv. Sci. 2019, 7, 1902295.
C. Becher, V. Laute, S. Fickert, W. Zinser, P. Niemeyer, T. John, P. Diehl, T. Kolombe, R. Siebold, J. Fay, J. Orthop. Surg. Res. 2017, 12, 71.
M. Krill, N. Early, J. S. Everhart, D. C. Flanigan, JBJS Rev. 2018, 6, e5.
N.-Y. Choi, B.-W. Kim, W.-J. Yeo, H.-B. Kim, D.-S. Suh, J.-S. Kim, Y.-S. Kim, Y.-H. Seo, J.-Y. Cho, C.-W. Chun, H.-S. Park, A. A. Shetty, S.-J. Kim, BMC Musculoskeletal Disord. 2010, 11, 103.
S. Xiao, T. Zhao, J. Wang, C. Wang, J. Du, L. Ying, J. Lin, C. Zhang, W. Hu, L. Wang, K. Xu, Stem Cell Rev. Rep. 2019, 15, 664.
R. Levato, W. R. Webb, I. A. Otto, A. Mensinga, Y. Zhang, M. van Rijen, R. van Weeren, I. M. Khan, J. Malda, Acta Biomater. 2017, 61, 41.
B. B. Rothrauff, K. Shimomura, R. Gottardi, P. G. Alexander, R. S. Tuan, Acta Biomater. 2017, 49, 140.
S. Žigon-Branc, M. Markovic, J. Van Hoorick, S. Van Vlierberghe, P. Dubruel, E. Zerobin, S. Baudis, A. Ovsianikov, Tissue Eng., Part A 2019, 25, 1369.
A. X. Sun, H. Lin, M. R. Fritch, H. Shen, P. G. Alexander, M. DeHart, R. S. Tuan, Acta Biomater. 2017, 58, 302.
L. Bian, M. Guvendiren, R. L. Mauck, J. A. Burdick, C. Hou, E. Tous, R. Rai, R. L. Mauck, J. A. Burdick, M. B. Fisher, Biomaterials 2013, 34, 413.
H. S. Cheung, Connect. Tissue Res. 1987, 16, 343.
W. K. Tam, A. De Vries, K. Cheung, G. Zhou, V. Leung, Global Spine J. 2014, 4, s-0034-1376607.
J. E. Lafont, S. Talma, C. Hopfgarten, C. L. Murphy, J. Biol. Chem. 2008, 283, 4778.
L. I. Smith-Mungo, H. M. Kagan, Matrix Biol. 1998, 16, 387.
C. Baugé, O. Cauvard, S. Leclercq, P. Galéra, K. Boumédiene, Arthritis Res. Ther. 2011, 13, R23.
R. T. Ballock, R. J. OʼKeefe, J. Bone Jt. Surg. 2003, 85, 715.
P. Zhuang, W. L. Ng, J. An, C. K. Chua, L. P. Tan, PLoS One 2019, 14, e0216776.
V. H. M. Mouser, F. P. W. Melchels, J. Visser, W. J. A. Dhert, D. Gawlitta, J. Malda, Biofabrication 2016, 8, 035003.
S. Afewerki, A. Sheikhi, S. Kannan, S. Ahadian, A. Khademhosseini, Bioeng. Transl. Med. 2019, 4, 96.
M. A. Woodruff, D. W. Hutmacher, Prog. Polym. Sci. 2010, 35, 1217.
R. Schipani, S. Scheurer, R. Florentin, S. E. Critchley, D. J. Kelly, Biofabrication 2020, 12, 035011.
M. Castilho, M. de Ruijter, S. Beirne, C. C. Villette, K. Ito, G. G. Wallace, J. Malda, Trends Biotechnol. 2020, 38, 1316.
P. Diloksumpan, M. de Ruijter, M. Castilho, U. Gbureck, T. Vermonden, P. R. van Weeren, J. Malda, R. Levato, Biofabrication 2020, 12, 025014.
E. Gevaert, L. Dollé, T. Billiet, P. Dubruel, L. van Grunsven, A. van Apeldoorn, R. Cornelissen, PLoS One 2014, 9, e105171.
L. De Moor, I. Merovci, S. Baetens, J. Verstraeten, P. Kowalska, D. V. Krysko, W. H. De Vos, H. Declercq, Biofabrication 2018, 10, 035009.
L. Tytgat, L. Van Damme, J. Van Hoorick, H. Declercq, H. Thienpont, H. Ottevaere, P. Blondeel, P. Dubruel, S. Van. Vlierberghe, Acta Biomater. 2019, 94, 340.
J. Van Hoorick, P. Gruber, M. Markovic, M. Tromayer, J. Van Erps, H. Thienpont, R. Liska, A. Ovsianikov, P. Dubruel, S. Van Vlierberghe, Biomacromolecules 2017, 18, 3260.