Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison.

Crystallography, X-Ray Proteins Synchrotrons

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
03 2021
Historique:
received: 07 10 2020
accepted: 28 01 2021
entrez: 18 3 2021
pubmed: 19 3 2021
medline: 19 4 2022
Statut: epublish

Résumé

For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.

Identifiants

DOI: 10.1126/sciadv.abf1380 PMID: 33731353 PMC: PMC7968842
pubmed: 33731353
pii: 7/12/eabf1380
doi: 10.1126/sciadv.abf1380
pmc: PMC7968842
pii:
doi:

Substances chimiques

Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Auteurs

P Mehrabi (P)

Department for Atomically Resolved Dynamics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany. pedram.mehrabi@mpsd.mpg.de eike.schulz@mpsd.mpg.de.
Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
Campbell Family Cancer Research Institute, Ontario Cancer Institute, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761 Hamburg, Germany.
ORCID: 0000-0003-3211-6959

R Bücker (R)

Department for Atomically Resolved Dynamics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
Centre for Structural Systems Biology, Department of Chemistry, University of Hamburg, Notkestraße 85, 22607 Hamburg, Germany.
ORCID: 0000-0001-7654-4707

G Bourenkov (G)

European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen-Synchrotron (DESY), Notkestraße 85, D-22603 Hamburg, Germany.
ORCID: 0000-0002-2617-5920

H M Ginn (HM)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.

D von Stetten (D)

European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen-Synchrotron (DESY), Notkestraße 85, D-22603 Hamburg, Germany.
ORCID: 0000-0001-7906-9788

H M Müller-Werkmeister (HM)

Institute of Chemistry-Physical Chemistry, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, Germany.
ORCID: 0000-0001-9471-882X

A Kuo (A)

Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
ORCID: 0000-0002-4234-6320

T Morizumi (T)

Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
ORCID: 0000-0002-2785-209X

B T Eger (BT)

Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
ORCID: 0000-0003-3350-9332

W-L Ou (WL)

Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
ORCID: 0000-0002-8069-5927

S Oghbaey (S)

Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.
ORCID: 0000-0003-3580-655X

A Sarracini (A)

Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.

J E Besaw (JE)

Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.
ORCID: 0000-0002-7571-7132

O Pare-Labrosse (O)

Department for Atomically Resolved Dynamics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.
ORCID: 0000-0001-9241-8356

S Meier (S)

Department of Physics, Universität Hamburg, Jungiusstrasse 9, 20355 Hamburg, Germany.

H Schikora (H)

Scientific Support Unit Machine Physics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
ORCID: 0000-0002-4778-6982

F Tellkamp (F)

Scientific Support Unit Machine Physics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
ORCID: 0000-0002-5670-3544

A Marx (A)

Department for Atomically Resolved Dynamics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761 Hamburg, Germany.

D A Sherrell (DA)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.
Structural Biology Center, X-ray Science Division, Argonne National Laboratory, Argonne, IL, USA.
ORCID: 0000-0002-6393-8800

D Axford (D)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.
ORCID: 0000-0001-7694-8525

R L Owen (RL)

Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, UK.
ORCID: 0000-0002-2104-7057

O P Ernst (OP)

Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
ORCID: 0000-0002-8863-9444

E F Pai (EF)

Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
Campbell Family Cancer Research Institute, Ontario Cancer Institute, 101 College Street, Toronto, Ontario M5G 1L7, Canada.
Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario M5S 1A8, Canada.
ORCID: 0000-0002-1162-7242

E C Schulz (EC)

Department for Atomically Resolved Dynamics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany. pedram.mehrabi@mpsd.mpg.de eike.schulz@mpsd.mpg.de.
Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761 Hamburg, Germany.
ORCID: 0000-0002-1685-8605

R J D Miller (RJD)

Department for Atomically Resolved Dynamics, Max Planck Institute for the Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany.
Center for Free-Electron Laser Science, Luruper Chaussee 149, 22761 Hamburg, Germany.
Departments of Chemistry and Physics, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.
Department of Physics, Universität Hamburg, Jungiusstrasse 9, 20355 Hamburg, Germany.
ORCID: 0000-0003-0884-0541

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Serial femtosecond and serial synchrotron...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Serial femtosecond and serial synchrotron...
questionsmedicales.fr › Équipement et fournitures › Équipement et fournitures pour les applications des radiations › Accélérateurs de particules › Synchrotrons › Serial femtosecond and serial synchrotron...