Tiletamine/zolazepam and dexmedetomidine with tramadol provide effective general anesthesia in rats.
Analgesics, Opioid
/ pharmacology
Anesthesia, General
/ veterinary
Anesthetics
/ pharmacology
Animals
Body Temperature
/ drug effects
Dexmedetomidine
/ pharmacology
Drug Combinations
Female
Heart Rate
/ drug effects
Hypnotics and Sedatives
/ pharmacology
Male
Oxygen
/ analysis
Rats, Sprague-Dawley
Respiratory Rate
/ drug effects
Surgical Wound
Tiletamine
/ pharmacology
Tramadol
/ pharmacology
Zolazepam
/ pharmacology
anesthesia
dexmedetomidine
rats
tiletamine
tramadol
Journal
Animal models and experimental medicine
ISSN: 2576-2095
Titre abrégé: Animal Model Exp Med
Pays: United States
ID NLM: 101726292
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
20
07
2020
accepted:
25
11
2020
entrez:
19
3
2021
pubmed:
20
3
2021
medline:
20
3
2021
Statut:
epublish
Résumé
Tiletamine/zolazepam is a dissociative anesthetic combination commonly used in small animals but information is limited in rats. The alpha-2 agonist, dexmedetomidine, has gained popularity in laboratory animal anesthesia. Tramadol is a weak opioid mu agonist. The aim of this study was to assess whether the tiletamine/zolazepam/dexmedetomidine (ZD) combination effectively provides a surgical anesthesia plane comparable to tiletamine/zolazepam/dexmedetomidine with tramadol (ZDT) in a minor procedure in rats. Rats were induced with ZD or ZDT. After the loss of paw withdrawal, a small incision was made on the rats' left thighs as a surgical stimulus. Rats were maintained under a surgical anesthesia plane by assessing the loss of the paw withdrawal reflex for 45 minutes, then atipamezole was administered. Monitored anesthesia parameters included: (a) physiological parameters - pulse rate (PR), respiratory rate (RR), tissue oxygen saturation (%SpO PR was significantly lower at 10 minutes in ZD and 5 minutes in ZDT groups. No difference was observed for RR, %SpO These data suggest the ZD combination provides a surgical anesthesia plane comparable to ZDT in a rat incisional pain model.
Sections du résumé
Background
Tiletamine/zolazepam is a dissociative anesthetic combination commonly used in small animals but information is limited in rats. The alpha-2 agonist, dexmedetomidine, has gained popularity in laboratory animal anesthesia. Tramadol is a weak opioid mu agonist. The aim of this study was to assess whether the tiletamine/zolazepam/dexmedetomidine (ZD) combination effectively provides a surgical anesthesia plane comparable to tiletamine/zolazepam/dexmedetomidine with tramadol (ZDT) in a minor procedure in rats.
Methods
Rats were induced with ZD or ZDT. After the loss of paw withdrawal, a small incision was made on the rats' left thighs as a surgical stimulus. Rats were maintained under a surgical anesthesia plane by assessing the loss of the paw withdrawal reflex for 45 minutes, then atipamezole was administered. Monitored anesthesia parameters included: (a) physiological parameters - pulse rate (PR), respiratory rate (RR), tissue oxygen saturation (%SpO
Results
PR was significantly lower at 10 minutes in ZD and 5 minutes in ZDT groups. No difference was observed for RR, %SpO
Conclusion
These data suggest the ZD combination provides a surgical anesthesia plane comparable to ZDT in a rat incisional pain model.
Identifiants
pubmed: 33738435
doi: 10.1002/ame2.12143
pii: AME212143
pmc: PMC7954827
doi:
Substances chimiques
Analgesics, Opioid
0
Anesthetics
0
Drug Combinations
0
Hypnotics and Sedatives
0
tiletamine, zolazepam drug combination
0
Tiletamine
2YFC543249
Tramadol
39J1LGJ30J
Dexmedetomidine
67VB76HONO
Zolazepam
G1R474U58U
Oxygen
S88TT14065
Types de publication
Journal Article
Langues
eng
Pagination
40-46Informations de copyright
© 2021 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences.
Références
J Am Assoc Lab Anim Sci. 2017 Mar 1;56(2):142-147
pubmed: 28315642
Pharmacol Biochem Behav. 1992 Dec;43(4):1129-33
pubmed: 1475295
Vet Anaesth Analg. 2014 Nov;41(6):636-43
pubmed: 24962038
J Am Assoc Lab Anim Sci. 2016 Jan;55(1):83-8
pubmed: 26817984
PLoS One. 2018 Oct 29;13(10):e0199087
pubmed: 30372437
Am J Vet Res. 2012 Nov;73(11):1707-14
pubmed: 23106454
Drugs. 2000 Jul;60(1):139-76
pubmed: 10929933
Neurotox Res. 2017 Nov;32(4):544-554
pubmed: 28577066
Comp Med. 2010 Aug;60(4):256-62
pubmed: 20819374
J Zoo Wildl Med. 2015 Sep;46(3):482-90
pubmed: 26352951
J Am Assoc Lab Anim Sci. 2007 Mar;46(2):74-80
pubmed: 17343357
Drug Saf. 1998 Sep;19(3):173-89
pubmed: 9747665
Vet Anaesth Analg. 2005 May;32(3):117-27
pubmed: 15877658
Lab Anim Sci. 1983 Oct;33(5):457-60
pubmed: 6645390
Lab Anim (NY). 2011 Mar;40(3):85-93
pubmed: 21326189
Anesthesiol Clin North Am. 2005 Mar;23(1):185-202
pubmed: 15763418
Pharmacol Ther. 2009 Aug;123(2):224-38
pubmed: 19393691
J Am Assoc Lab Anim Sci. 2011 Mar;50(2):192-7
pubmed: 21439212
J Am Assoc Lab Anim Sci. 2010 Jan;49(1):45-51
pubmed: 20122316
Comp Med. 2001 Oct;51(5):443-56
pubmed: 11924805
J Vet Sci. 2003 Aug;4(2):187-91
pubmed: 14610374
Biomed Pharmacother. 2019 Mar;111:443-451
pubmed: 30594783