The mechanism for brassinosteroids suppressing climacteric fruit ripening.
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
23 04 2021
23 04 2021
Historique:
received:
01
12
2020
accepted:
02
01
2021
pubmed:
21
3
2021
medline:
1
9
2021
entrez:
20
3
2021
Statut:
ppublish
Résumé
The plant hormone ethylene is important for the ripening of climacteric fruit, such as pear (Pyrus ussuriensis), and the brassinosteroid (BR) class of phytohormones affects ethylene biosynthesis during ripening via an unknown molecular mechanism. Here, we observed that exogenous BR treatment suppressed ethylene production and delayed fruit ripening, whereas treatment with a BR biosynthesis inhibitor promoted ethylene production and accelerated fruit ripening in pear, suggesting BR is a ripening suppressor. The expression of the transcription factor BRASSINAZOLE-RESISTANT 1PuBZR1 was enhanced by BR treatment during pear fruit ripening. PuBZR1 interacted with PuACO1, which converts 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene, and suppressed its activity. BR-activated PuBZR1 bound to the promoters of PuACO1 and of PuACS1a, which encodes ACC synthase, and directly suppressed their transcription. Moreover, PuBZR1 suppressed the expression of transcription factor PuERF2 by binding its promoter, and PuERF2 bound to the promoters of PuACO1 and PuACS1a. We concluded that PuBZR1 indirectly suppresses the transcription of PuACO1 and PuACS1a through its regulation of PuERF2. Ethylene production and expression profiles of corresponding apple (Malus domestica) homologs showed similar changes following epibrassinolide treatment. Together, these results suggest that BR-activated BZR1 suppresses ACO1 activity and the expression of ACO1 and ACS1, thereby reducing ethylene production and suppressing fruit ripening. This likely represents a conserved mechanism by which BR suppresses ethylene biosynthesis during climacteric fruit ripening.
Identifiants
pubmed: 33743010
pii: 6102590
doi: 10.1093/plphys/kiab013
pmc: PMC8133653
doi:
Substances chimiques
Brassinosteroids
0
Ethylenes
0
Plant Growth Regulators
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1875-1893Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.
Références
Plant Physiol. 2016 Jun;171(2):1070-84
pubmed: 27208241
Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7905-9
pubmed: 10393920
PLoS One. 2014 Sep 12;9(9):e107562
pubmed: 25215597
Biochemistry. 1997 Dec 16;36(50):15999-6007
pubmed: 9398335
Plant Physiol. 2006 Jan;140(1):150-8
pubmed: 16361521
Plant J. 2004 Nov;40(3):428-38
pubmed: 15469500
Phytochemistry. 2002 Dec;61(7):843-7
pubmed: 12453577
Plant Biotechnol J. 2016 Mar;14(3):1021-33
pubmed: 26383874
Plant Cell. 2017 Jun;29(6):1316-1334
pubmed: 28550149
J Plant Physiol. 2013 Jul 15;170(11):987-95
pubmed: 23507024
Science. 2005 Mar 11;307(5715):1634-8
pubmed: 15681342
Plant Cell. 2011 Apr;23(4):1219-30
pubmed: 21505068
Biochem Biophys Res Commun. 1996 Aug 23;225(3):697-700
pubmed: 8780676
J Exp Bot. 2009;60(6):1579-88
pubmed: 19246595
J Exp Bot. 2013 Feb;64(4):1049-59
pubmed: 23364941
Plant Cell. 2020 Feb;32(2):295-318
pubmed: 31776234
Plant Cell. 2002;14 Suppl:S97-110
pubmed: 12045272
Plant J. 2016 Dec;88(5):735-748
pubmed: 27476697
Cell. 2005 Jan 28;120(2):249-59
pubmed: 15680330
Planta. 1984 Mar;160(3):281-7
pubmed: 24258513
Plant Physiol. 2008 Feb;146(2):368-76
pubmed: 18065554
Transgenic Res. 2004 Aug;13(4):373-84
pubmed: 15517996
Annu Rev Plant Biol. 2010;61:681-704
pubmed: 20192752
Plant Physiol. 2013 Aug;162(4):2056-66
pubmed: 23800992
Plant Commun. 2020 Apr 21;1(3):100047
pubmed: 33367242
Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9789-93
pubmed: 1409700
Plant Cell. 2007 Sep;19(9):2749-62
pubmed: 17873094
Trends Plant Sci. 2007 Jan;12(1):37-41
pubmed: 17142084
PLoS Genet. 2018 Jan 11;14(1):e1007144
pubmed: 29324765
Annu Rev Genet. 2011;45:41-59
pubmed: 22060040
Plant J. 2008 Jul;55(2):212-23
pubmed: 18363783
Nat Plants. 2019 Mar;5(3):290-299
pubmed: 30833710
J Exp Bot. 2007;58(12):3299-308
pubmed: 17925301
Plant Physiol. 2007 Aug;144(4):1899-912
pubmed: 17556515
Front Plant Sci. 2013 Jun 14;4:198
pubmed: 23785378
J Agric Food Chem. 2018 Mar 21;66(11):2637-2644
pubmed: 29509414
J Exp Bot. 2013 May;64(8):2499-510
pubmed: 23599278