A dansyl-derivatized phytic acid analogue as a fluorescent substrate for phytases: experimental and computational approach.
Artificial substrate
Fertilizer
Inositol-phosphates
Kinetics
Phosphate
Phytases
Soil
Journal
Bioorganic chemistry
ISSN: 1090-2120
Titre abrégé: Bioorg Chem
Pays: United States
ID NLM: 1303703
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
10
12
2020
revised:
03
02
2021
accepted:
05
03
2021
pubmed:
22
3
2021
medline:
13
10
2021
entrez:
21
3
2021
Statut:
ppublish
Résumé
A new myo-inositol pentakisphosphate was synthesized, which featured a dansyl group at position C-5. The fluorescent tag was removed from the inositol by a 6-atom spacer to prevent detrimental steric interactions in the catalytic site of phytases. The PEG linker was used in order to enhance hydrophilicity and biocompatibility of the new artificial substrate. Computational studies showed a favorable positioning in the catalytic site of phytases. Enzymatic assays demonstrated that the tethered myo-inositol was processed by two recombinant phytases Phy-A and Phy-C, classified respectively as acid and alkaline phytases, with similar rates of phosphate release compared to their natural substrate.
Identifiants
pubmed: 33744806
pii: S0045-2068(21)00187-5
doi: 10.1016/j.bioorg.2021.104810
pii:
doi:
Substances chimiques
1-myristoyl-2-(12-((5-dimethylamino-1-naphthalenesulfonyl)amino)dodecanoyl)-sn-glycero-3-phosphocholine
0
Fluorescent Dyes
0
Phosphatidylcholines
0
Phytic Acid
7IGF0S7R8I
6-Phytase
EC 3.1.3.26
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104810Informations de copyright
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